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Table 3 Statistical analysis of Plackett-Burman design

From: Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Term Coefficient Effect % Contribution F-value P-value
Prob > F
Intercept 5.17    573.21 0.0001*
A-Temperature 0.15 0.30 0.25 17.15 0.0256*
B-Incubation time −0.88 −1.77 8.86 615.20 0.0001*
C-Inoculum size −0.45 −0.91 2.33 161.94 0.0010*
D-Agitation speed 1.01 2.02 11.58 804.04 <  0.0001*
E-pH 0.42 0.84 2.00 138.84 0.0013*
F-Glucose −0.78 −1.56 6.92 480.30 0.0002*
G-Starch 0.04 0.09 0.02 9.87 × 10−3 0.9271*
H-Cholesterol 2.18 4.37 54.13 3757.27 <  0.0001*
J-Peptone 0.02 0.05 0.01 3.38 × 10−3 0.9573
K-Yeast extract 0.95 1.90 10.20 707.66 0.0001*
L-(NH4)2SO4 0.04 0.07 0.02 7.37 × 10− 3 0.9370
M- K2HPO4 0.17 0.34 0.33 22.89 0.0174*
N-NaCl −0.41 −0.83 1.95 135.29 0.0014*
O- MgSO4.7H2O −0.11 − 0.23 0.14 9.98 0.0509
P- FeSO4.7H2O −0.19 −0.38 0.40 27.95 0.0132*
PRESS 12.92 Adeq Precision 79.34
C.V. % 3.08 Pred R2 0.9261
Mean 5.17 Adj R2 0.9978
Std. Dev. 0.16 R2 0.9996
  1. * Significant values, “F: Fishers’s function, PRESS is the predicted residual sum of squares, C.V. % is the coefficient of variation%”