Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Table 3 Statistical analysis of Plackett-Burman design

Term	Coefficient	Effect	% Contribution	F-value	P-value Prob > F
Intercept	5.17			573.21	0.0001*
A-Temperature	0.15	0.30	0.25	17.15	0.0256*
B-Incubation time	−0.88	−1.77	8.86	615.20	0.0001*
C-Inoculum size	−0.45	−0.91	2.33	161.94	0.0010*
D-Agitation speed	1.01	2.02	11.58	804.04	< 0.0001*
E-pH	0.42	0.84	2.00	138.84	0.0013*
F-Glucose	−0.78	−1.56	6.92	480.30	0.0002*
G-Starch	0.04	0.09	0.02	9.87 × 10⁻³	0.9271*
H-Cholesterol	2.18	4.37	54.13	3757.27	< 0.0001*
J-Peptone	0.02	0.05	0.01	3.38 × 10⁻³	0.9573
K-Yeast extract	0.95	1.90	10.20	707.66	0.0001*
L-(NH₄)₂SO₄	0.04	0.07	0.02	7.37 × 10^− 3	0.9370
M- K₂HPO₄	0.17	0.34	0.33	22.89	0.0174*
N-NaCl	−0.41	−0.83	1.95	135.29	0.0014*
O- MgSO₄.7H₂O	−0.11	− 0.23	0.14	9.98	0.0509
P- FeSO₄.7H₂O	−0.19	−0.38	0.40	27.95	0.0132*
PRESS	12.92		Adeq Precision		79.34
C.V. %	3.08		Pred R²		0.9261
Mean	5.17		Adj R²		0.9978
Std. Dev.	0.16		R²		0.9996

* Significant values, “F: Fishers’s function, PRESS is the predicted residual sum of squares, C.V. % is the coefficient of variation%”

ISSN: 1471-2180