Table 2 Twenty-trial Plackett–Burman experimental design used for cholesterol oxidase production
Std | Run no. | Coded levels of independent variables | Cholesterol oxidase activity (U/mL) | Residuals | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Temperature (°C) | Incubation time (Days) | Inoculum size % (v/v) | Agitation speed (rpm) | pH | Glucose (g/L) | Starch (g/L) | Cholesterol (g/L) | Peptone (g/L) | Yeast extract (g/L) | (NH4)2SO4(g/L) | K2HPO4(g/L) | NaCl(g/L) | MgSO4.7H2O(g/L) | FeSO4.7H2O(g/L) | D1 | D2 | D3 | D4 | Actual value | Predicted value | |||
6 | 1 | −1 | −1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | -1 | 1 | -1 | 1 | -1 | -1 | 3.39 | 3.71 | −0.32 |
12 | 2 | −1 | 1 | − 1 | 1 | − 1 | − 1 | − 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | − 1 | − 1 | 1 | 1 | 1 | 1 | 4.60 | 4.78 | −0.18 |
4 | 3 | 1 | 1 | −1 | 1 | 1 | − 1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | − 1 | −1 | − 1 | 9.72 | 9.57 | 0.15 |
15 | 4 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | − 1 | − 1 | −1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 0.87 | 0.72 | 0.15 |
20 | 5 | −1 | −1 | − 1 | − 1 | −1 | − 1 | −1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | −1 | − 1 | −1 | − 1 | −1 | 3.15 | 3.12 | 0.03 |
7 | 6 | −1 | −1 | − 1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | 10.11 | 9.64 | 0.47 |
2 | 7 | −1 | 1 | 1 | − 1 | − 1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | −1 | − 1 | −1 | 1 | 1 | 2.82 | 2.43 | 0.39 |
18 | 8 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | − 1 | −1 | − 1 | 1 | 1 | −1 | 1 | 1 | 9.67 | 9.40 | 0.27 |
16 | 9 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | − 1 | − 1 | − 1 | − 1 | 1 | 1 | −1 | 1 | 1 | − 1 | −1 | 4.12 | 4.52 | −0.39 |
10 | 10 | −1 | 1 | −1 | − 1 | −1 | − 1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | 6.74 | 7.02 | − 0.28 |
14 | 11 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | − 1 | − 1 | −1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 2.11 | 1.85 | 0.26 |
1 | 12 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | −1 | − 1 | −1 | 1 | 1 | −1 | 7.35 | 7.53 | −0.19 |
17 | 13 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | −1 | − 1 | −1 | 1 | 1 | −1 | 1 | 1 | −1 | 2.57 | 2.71 | −0.14 |
8 | 14 | −1 | −1 | −1 | − 1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | 5.21 | 5.68 | −0.47 |
11 | 15 | 1 | −1 | 1 | −1 | − 1 | −1 | − 1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 6.78 | 6.84 | −0.07 |
5 | 16 | −1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | − 1 | −1 | 1.97 | 1.74 | 0.23 |
19 | 17 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | − 1 | −1 | − 1 | 1 | 1 | −1 | 1 | 4.49 | 4.72 | −0.23 |
3 | 18 | 1 | −1 | 1 | 1 | −1 | − 1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | −1 | −1 | − 1 | −1 | 1 | 11.03 | 10.91 | 0.12 |
13 | 19 | 1 | −1 | 1 | −1 | 1 | −1 | −1 | − 1 | −1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 3.95 | 4.19 | −0.24 |
9 | 20 | 1 | −1 | −1 | −1 | − 1 | 1 | 1 | −1 | 1 | 1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | −1 | 2.77 | 2.32 | 0.45 |
Variable level | A | B | C | D | E | F | G | H | J | K | L | M | N | O | P | ||||||||
−1 | 30 | 5 | 2 | 100 | 7 | 5 | 5 | 1 | 1 | 1 | 4 | 0.5 | 0.5 | 0.1 | 0.01 | ||||||||
1 | 37 | 7 | 4 | 150 | 9 | 10 | 10 | 3 | 4 | 4 | 8 | 1.0 | 1.0 | 0.5 | 0.05 | ||||||||