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Table 2 Twenty-trial Plackett–Burman experimental design used for cholesterol oxidase production

From: Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Std Run no. Coded levels of independent variables Cholesterol oxidase activity (U/mL) Residuals
Temperature (°C) Incubation time (Days) Inoculum size % (v/v) Agitation speed (rpm) pH Glucose (g/L) Starch (g/L) Cholesterol (g/L) Peptone (g/L) Yeast extract (g/L) (NH4)2SO4(g/L) K2HPO4(g/L) NaCl(g/L) MgSO4.7H2O(g/L) FeSO4.7H2O(g/L) D1 D2 D3 D4 Actual value Predicted value
6 1 −1 −1 1 1 −1 1 1 −1 −1 1 1 1 1 -1 1 -1 1 -1 -1 3.39 3.71 −0.32
12 2 −1 1 − 1 1 − 1 − 1 − 1 − 1 1 1 − 1 1 1 − 1 − 1 1 1 1 1 4.60 4.78 −0.18
4 3 1 1 −1 1 1 − 1 −1 1 1 1 1 −1 1 −1 1 −1 − 1 −1 − 1 9.72 9.57 0.15
15 4 1 1 1 −1 1 −1 1 −1 − 1 − 1 −1 1 1 −1 1 1 −1 −1 1 0.87 0.72 0.15
20 5 −1 −1 − 1 − 1 −1 − 1 −1 − 1 − 1 − 1 − 1 − 1 − 1 − 1 −1 − 1 −1 − 1 −1 3.15 3.12 0.03
7 6 −1 −1 − 1 1 1 −1 1 1 −1 −1 1 1 1 1 −1 1 −1 1 −1 10.11 9.64 0.47
2 7 −1 1 1 − 1 − 1 1 1 1 1 −1 1 −1 1 −1 −1 − 1 −1 1 1 2.82 2.43 0.39
18 8 −1 −1 1 1 1 1 −1 1 −1 1 −1 − 1 −1 − 1 1 1 −1 1 1 9.67 9.40 0.27
16 9 1 1 1 1 −1 1 −1 1 − 1 − 1 − 1 − 1 1 1 −1 1 1 − 1 −1 4.12 4.52 −0.39
10 10 −1 1 −1 − 1 −1 − 1 1 1 −1 1 1 −1 −1 1 1 1 1 −1 1 6.74 7.02 − 0.28
14 11 1 1 −1 1 −1 1 −1 − 1 − 1 −1 1 1 −1 1 1 −1 −1 1 1 2.11 1.85 0.26
1 12 1 1 −1 −1 1 1 1 1 −1 1 −1 1 −1 −1 − 1 −1 1 1 −1 7.35 7.53 −0.19
17 13 −1 1 1 1 1 −1 1 −1 1 −1 −1 − 1 −1 1 1 −1 1 1 −1 2.57 2.71 −0.14
8 14 −1 −1 −1 − 1 1 1 −1 1 1 −1 −1 1 1 1 1 −1 1 −1 1 5.21 5.68 −0.47
11 15 1 −1 1 −1 − 1 −1 − 1 1 1 −1 1 1 −1 −1 1 1 1 1 −1 6.78 6.84 −0.07
5 16 −1 1 1 −1 1 1 −1 −1 1 1 1 1 −1 1 −1 1 −1 − 1 −1 1.97 1.74 0.23
19 17 1 −1 −1 1 1 1 1 −1 1 −1 1 −1 − 1 −1 − 1 1 1 −1 1 4.49 4.72 −0.23
3 18 1 −1 1 1 −1 − 1 1 1 1 1 −1 1 −1 1 −1 −1 − 1 −1 1 11.03 10.91 0.12
13 19 1 −1 1 −1 1 −1 −1 − 1 −1 1 1 −1 1 1 −1 −1 1 1 1 3.95 4.19 −0.24
9 20 1 −1 −1 −1 − 1 1 1 −1 1 1 −1 −1 1 1 1 1 −1 1 −1 2.77 2.32 0.45
Variable level A B C D E F G H J K L M N O P        
−1 30 5 2 100 7 5 5 1 1 1 4 0.5 0.5 0.1 0.01        
1 37 7 4 150 9 10 10 3 4 4 8 1.0 1.0 0.5 0.05        
  1. “Std” is the standard order and “D” means dummy