Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Table 1 Phenotypic properties of Streptomyces sp. strain NEAE-94 and related Streptomyces species. Reference species properties have been taken from Bergey’s Manual of Systematic Bacteriology [20]

Characteristic	*Streptomyces* sp. strain NEAE-94	*Streptomyces anulatus*	*Streptomyces flavofuscus*	*Streptomyces fimicarius*	*Streptomyces parvus*
Aerial mycelium on ISP medium 2	Whitish -yellow	Yellow or white	Yellow, green-yellow	Yellow or white	Yellow
Diffusible pigment	Yellow	Trace of yellow	Yellow-gray-brown		Yellow
Substrate mycelium on ISP medium 2	Yellow	Pale yellow; grayish yellow or yellowish brown	Yellow- gray-brown	Not distinctive	Not distinctive
Surface of spores	Smooth	Smooth	Smooth	Smooth	Smooth
Morphology of spore chain	Rectiflexibles	Rectiflexibiles	Rectiflexibiles	Rectiflexibiles	Rectiflexibiles
Melanin production on Tyrosine agar, peptone-yeast extract iron agar or tryptone-yeast extract broth	–	–	–	–	–
Utilization of carbon sources
D(+) glucose, D(−) fructose, L-arabinose, D(+) xylose, rhamnose	+	+		+	+
Sucrose, raffinose	+	+		±	±
D(+) mannose, cellulose	+	+		+
Trehalose, D(+) galactose, maltose	+
Ribose	–

Abbreviations: + Positive, − Negative, ± Doubtful, Blank cells no data available. L-asparaginase, gelatinase, cellulose, protease and α–amylase were produced by Streptomyces sp. strain NEAE-94 while chitosanase, lecithinase and uricase were not produced. Maximum NaCl tolerance (5%, w/v).The optimal growth temperature was 30°C and optimal pH was 7.0. It exhibited positive antimicrobial activities against Staphylococcus aureus, E. coli, Bacillus subtilis and Pseudomonas aeruginosa. Nitrate reduction, coagulation and peptonization of milk were positive

ISSN: 1471-2180