Fig. 7

A majority of tested CPSR and VSR genes are expressed in innate immune response tissues. Expression of several CPSR (T24B8.5, F19B2.5, K08D8.4) and VSR (sodh-1, acdh-1, dhs-3) genes using transcriptional or translational fluorescent protein fusions upon exposure to S. maltophilia or E. coli OP50. Up- or down- arrows below each gene name indicate direction of expression in reference to the pathogenic strains (up/down-regulated in response to JV3 and/or JCMS). All transgenes are primarily expressed in the intestine, except for sodh-1, which is also expressed in several other tissues. Columns show expression of worms exposed to E. coli OP50 and S. maltophilia K279a, JCMS, or JV3 for 24 h at 100x magnification. The images chosen represent mean average intensity across 10–18 worms (see Additional File 8). Scale bar indicates 100 μm, anterior is to the left, and ventral is up for all pictures. Note that expression in the AIY interneuron in the T24B8.5 transgenic strain is due to a ttx-3:GFP marker and not T24B8.5 expression and the sodh-1 expression construct contains a nuclear localization signal