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Table 1 BOXA-PCR fragment sequencing and confirmatory results

From: Bacteriophage genotyping using BOXA repetitive-PCR

FRAGMENT NAME
(Primer)
Rep-PCR
fragment size-gel assessment (bp)
UniProt KB annotation of rep-PCR fragment
(Top BLAST result)
Rep-PCR
BLAST results
Confirmatory BLAST results using primers designed to amplify the putative CDS*
ØX174-1
(BOXA1R)
650 P03631 Replication-associated protein A (Enterobacteria phage phiX174)a E-value: 1.9e-91
Score: 745
Ident.: 89.1%
E-value: 0.0
Score: 1819
Ident.: 93.30%
ØT4-2
(BOXA2R)
700 A0A376YLU8 Putative baseplate structural protein (Escherichia coli) E-value: 1.7e-13
Score: 188
Ident.: 36.7%
E-value: 3.9e-133
Score: 1004
Ident.: 98.50%
ØLambda-1
(BOXA2R)
450 P03689 Replication protein P (Escherichia phage lambda)a E-value: 1e-15
Score: 166
Ident.: 43.2%
E-value: 3.6e-156
Score: 1133
Ident.: 100.00%
Øc2-1
(BOXA1R)
1200 Q38305 Probable tape measure protein (Lactococcus phage c2) E-value: 5.8e-17
Score: 145
Ident.: 66.7%
E-value: 0.0
Score: 1529
Ident.: 94.70%
  1. *refer to Tables 1 and 2 (Additional file 5) for details