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Table 1 BOXA-PCR fragment sequencing and confirmatory results

From: Bacteriophage genotyping using BOXA repetitive-PCR

FRAGMENT NAME

(Primer)

Rep-PCR

fragment size-gel assessment (bp)

UniProt KB annotation of rep-PCR fragment

(Top BLAST result)

Rep-PCR

BLAST results

Confirmatory BLAST results using primers designed to amplify the putative CDS*

ØX174-1

(BOXA1R)

650

P03631 Replication-associated protein A (Enterobacteria phage phiX174)a

E-value: 1.9e-91

Score: 745

Ident.: 89.1%

E-value: 0.0

Score: 1819

Ident.: 93.30%

ØT4-2

(BOXA2R)

700

A0A376YLU8 Putative baseplate structural protein (Escherichia coli)

E-value: 1.7e-13

Score: 188

Ident.: 36.7%

E-value: 3.9e-133

Score: 1004

Ident.: 98.50%

ØLambda-1

(BOXA2R)

450

P03689 Replication protein P (Escherichia phage lambda)a

E-value: 1e-15

Score: 166

Ident.: 43.2%

E-value: 3.6e-156

Score: 1133

Ident.: 100.00%

Øc2-1

(BOXA1R)

1200

Q38305 Probable tape measure protein (Lactococcus phage c2)

E-value: 5.8e-17

Score: 145

Ident.: 66.7%

E-value: 0.0

Score: 1529

Ident.: 94.70%

  1. *refer to Tables 1 and 2 (Additional file 5) for details