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Table 3 Plasmids and bacterial strains constructed in the study

From: Protein–protein interaction of Rv0148 with Htdy and its predicted role towards drug resistance in Mycobacterium tuberculosis

PlasmidSource of descriptionReference/origin
E. coli DH5αFendA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+), λ-Invitrogen
E. coli BL21 (DE3)FompT gal dcm lon hsdSB(rBmB) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]Invitrogen
M. smegmatis mc2155Lab stock 
pGEX 4 T-1lacTq, 4.9 kb, Ampr, GST gene fusion vectorGE Healthcare
pRSET-BlacTq, 2.8 kb, AmpR, 6xHis (N-term) gene fusion vectorInvitrogen
p0004- Sac BSuicide recombination delivery vector carrying hygR-SacB for gene disruption, hygR 
PhAE159Conditionally replicating shuttle phasmid vectorWilliam R Jacobs Jr (unpublished)
pMV261E. coli mycobacterial shuttle vector, KanR, hsp60 promoterStrover et al. (1991)
Constructs in this study
 pGBRJ1pRSET-B vector carrying the coding region of Rv0148 gene from M. tuberculosis, AmpRThis study
 pGBRJ2pGEX 4 T-1 vector carrying the coding region of Rv0148 gene from M. tuberculosis, AmpRThis study
 pGBRJ3pRSET-B vector carrying the coding region of Htdy gene from M. tuberculosis, AmpRThis study
 Δ0148The deletion of Rv0148 gene from M. tuberculosisThis study
 CΔ0148pMV261 vector carrying the coding region of Rv0148 gene from M. tuberculosis, KanR expressed in M. tuberculosis (complementation)This study