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Fig. 4 | BMC Microbiology

Fig. 4

From: Protein–protein interaction of Rv0148 with Htdy and its predicted role towards drug resistance in Mycobacterium tuberculosis

Fig. 4

Rv0148 interacts with Htdy. Pull-down assay confirms the interaction between Rv0148 and Htdy. The crude lysates enclosing His-Htdy and GST-Rv0148 were incubated with Ni-NTA resin; unbound proteins (lane 1) were washed off and incubated with their corresponding substrates. The protein fractions were eluted (lanes 2 and 4) and immunoblotted with anti-His and anti-GST antibodies. As a control, the bait proteins were incubated with bovine serum albumin protein (lane 3). Lane M, molecular mass standard

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