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Fig. 2 | BMC Microbiology

Fig. 2

From: Vibiro vulnificus hemolysin associates with gangliosides

Fig. 2

Gangliosides inhibit cytotoxicity by preventing VVH binding. a Inhibition of VVH-induced cytotoxicity by various gangliosides. VVH were preincubated with various gangliosides at the indicated molar ratio (VVH:ganglioside). The percentages of LDH release were calculated as described in the Materials and Methods. **; Significant decrease compared with the LDH release of VVH without ganglioside (ANOVA and Tukey’s test, P < 0.01). b Prevention assay for binding of VVH on CHO cells by ganglioside. VVH was preincubated with Gg4Cer or GD1a as the indicated molar ratio. Binding of VVH and cellular actin were detected by using appropriate antibodies as described in Materials and Methods

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