TY - JOUR AU - Martinez, Stacy AU - Garcia, Jonathan Gomez AU - Williams, Roy AU - Elmassry, Moamen AU - West, Andrew AU - Hamood, Abdul AU - Hurtado, Deborah AU - Gudenkauf, Brent AU - Ventolini, Gary AU - Schlabritz-Loutsevitch, Natalia PY - 2020 DA - 2020/03/24 TI - Lactobacilli spp.: real-time evaluation of biofilm growth JO - BMC Microbiology SP - 64 VL - 20 IS - 1 AB - Biofilm is a fundamental bacterial survival mode which proceeds through three main generalized phases: adhesion, maturation, and dispersion. Lactobacilli spp. (LB) are critical components of gut and reproductive health and are widely used probiotics. Evaluation of time-dependent mechanisms of biofilm formation is important for understanding of host-microbial interaction and development of therapeutic interventions. Time-dependent LB biofilm growth was studied in two systems: large biofilm output in continuous flow system (microfermenter (M), Institute Pasteur, France) and electrical impedance-based real time label-free cell analyzer (C) (xCELLigence, ACEA Bioscience Inc., San Diego, CA). L. plantarum biofilm growth in M system was video-recorded, followed by analyses using IMARIS software (Bitplane, Oxford Instrument Company, Concord, MA, USA). Additionally, whole genome expression and analyses of attached (A) and dispersed (D) biofilm phases at 24 and 48 h were performed. SN - 1471-2180 UR - https://doi.org/10.1186/s12866-020-01753-3 DO - 10.1186/s12866-020-01753-3 ID - Martinez2020 ER -