Table 3 List of oligonucleotides used in this study

pET28b_Fw

GACTCACTATAGGGGAATTGTGAGCG

pET28b_Rv

AGATCCGGCTGCTAACAAAGCCCGA

pVWEx1_fw

CACTCCCGTTCTGGATAATG

pVWEx1_rv

GCTACGGCGTTTCACTTCTG

pTH1_fw

CTGCCCTTCCACCTTAACC

pTH1_rv

ATGTCACTAACCTGCCCCG

ta_MGA3rec-RBS-fw

GCGCGGATCCGAAAGGAGGCCCTTCAGATGGATGATTCAAAACAGTT

ta_MGA3rec-rv

GCGCGGTACCTTATTTCCCGCGTTTATTCC

ta_PB1-RBS-fw

GCGCGGATCCGAAAGGAGGCCCTTCAGATGATTCAAAACAGCATCCA

ta_PB1-rv

GCGCGGTACCTTATTGCCCGCGTTTTTTCC

tal_CG-RBS-fw

GCGCGGATCCGAAAGGAGGCCCTTCAGATGTCTCACATTGATGATCT

tal_CG-rv

GCGCGGTACCCTACTTCAGGCGAGCTTCCA

ta_PB1-TH-fw

GCGCACATGTGATTCAAAACAGCATCCAACGAAAT

ta_PB1-TH-rv,

ATGCGGTACCTTATTGCCCGCGTTTTTTCC

ta_PB1_NcoI-fw

AGAGCCATGGATGATTCAAAACAGCATCCA

ta_PB1_Xho-rv

AGAGCTCGAGTTGCCCGCGTTTTTTCCAATCTG

ta_MGA3rec_NcoI-fw

AGAGCCATGGATGATTCAAAACAGTTTCCAACCAAATAAAG

ta_MGA3rec_Xho-rv

AGAGCTCGAGTTTCCCGCGTTTATTCCAGTC

tal_cg_del_A

GCGCGGATCCGGCTCCGGCTCCGAGGTTCA

tal_cg_del_B

CCCATCCACTAAACTTAAACAGAGCTGTGCAAGATCATCAA

tal_cg_del_C

TGTTTAAGTTTAGTGGATGGGCTTGAGTCCATGGAAGCTCG

tal_cg_del_D

GCGCGGATCCGCGGGTTTTGTCGATGCGCT

tal_cg_del_E

CTGCGTCCTGCAGATGCGAA

tal_cg_del_F

GGTCGATGCGGAACACAGAA

ta_MGA3,M1_BamHI-fw

CGCGGATCCGTGTCATATTTTAATACAGTT

ta_MGA3,M2_BamHI-fw

CGCGGATCCGTGTCAAAATTTAATACAGTT

ta_MGA3,M3_BamHI-fw

CGCGGATCCGTGTCATTATTTAATACAGTT

ta_MGA3,M4_BamHI-fw

CGCGGATCCGTGTCATCATTTAATACAGTT

ta_MGA3,M5_BamHI-fw

CGCGGATCCGTGTCACAATTTAATACAGTT

ta_MGA3,M6_BamHI-fw

CGCGGATCCGTGTCAGAATTTAATACAGTT

ta_MGA3,M_BamHI-rv

CCCCGGATCCTTATTTCCCGCGTTTATTCC

ta_MGA3_fw

ta_MGA3_rv