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Table 2 List of bacterial strains and plasmids used in this study

From: Transaldolase in Bacillus methanolicus: biochemical characterization and biological role in ribulose monophosphate cycle

Strain, plasmidFunction and relevant characteristicsReferences
B. methanolicusstrains
 MGA3Wild type strain, ATCC 53907[53]
 PB1Wild type strain, NCIMBI 13113[53]
E. colistrains
 DH5αGeneral cloning host. Fthi-1 endA1 hsdR17(r m) supE44 ΔlacU169 (80lacZΔM15) recA1 gyrA96 relA1Bethesda Research Laboratories
 BL21protein expression host. ompT hsdSB (rB mB_) gal dcm (DE3)Novagen
C. glutamicumstrains
 ATCC 13032WT strain, auxotrophic for biotin[1]
ΔtalIn-frame deletion of the tal gene of WTThis study
Plasmids
 pVWEx1KmR; C. glutamicum-E. coli shuttle vector (PtaclacIqoriVC.goriVE.c)[46]
 pVWEx1-taPB1derived from pVWEx1, for regulated expression of tal of B. methanolicus PB1This study
 pVWEx1-taMGA3recderived from pVWEx1, for regulated expression of modified tal of B. methanolicus MGA3This study
 pVWEx1-talCGderived from pVWEx1, for regulated expression of tal (cg1776) of C. glutamicum ATCC 13032This study
 pHP13B. subtilis-E coli shuttle vector; ClmR[22]
 pTH1mp-taPB1pHP13 derivate with tal of B. methanolicus PB1 under control of mdh promoterThis study
 pET28bKanR; T7lac; vector for his-tagged protein overproduction(Novagen)
 pET28b-taPB1purification of his-tagged B. methanolicus PB1 Ta from E. coli BL21(DE3)This study
 pET28b-taMGA3recpurification of his-tagged modified B. methanolicus MGA3 Ta from E. coli BL21(DE3)This study
 pET28b-taMGA3_M1derived from pET28b, for expression of variant M1 of taMGA3 from B. methanolicus MGA3, see Table 3This study
 pET28b-taMGA3_M2derived from pET28b, for expression of variant M2 of taMGA3 from B. methanolicus MGA3, see Table 3This study
 pET28b-taMGA3_M3derived from pET28b, for expression of variant M3 of taMGA3 from B. methanolicus MGA3, see Table 3This study
 pET28b-taMGA3_M4derived from pET28b, for expression of variant M4 of taMGA3 from B. methanolicus MGA3, see Table 3This study
 pET28b-taMGA3_M5derived from pET28b, for expression of variant M5 of taMGA3 from B. methanolicus MGA3, see Table 3This study
 pET28b-taMGA3_M6derived from pET28b, for expression of variant M6 of taMGA3 from B. methanolicus MGA3, see Table 3This study
 pET28b-taMGA3derived from pET28b, for expression of variant of the annotated taMGA3 from B. methanolicus MGA3, see Table 3 
 pK19mobsacBKanR; vector for gene deletions (RP4 mob; sacB B. subtilis; lacZ; OriVE.c.)[52]
 pK19mobsacB-Δtalderived from pK19mobsacB for in-frame deletion of C. glutamicum talThis study
  1. Abbreviations: SpeR, spectinomycin resistance; CmR, chloramphenicol resistance; KanR kanamycin resistance