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Table 2 List of bacterial strains and plasmids used in this study

From: Transaldolase in Bacillus methanolicus: biochemical characterization and biological role in ribulose monophosphate cycle

Strain, plasmid

Function and relevant characteristics

References

B. methanolicusstrains

 MGA3

Wild type strain, ATCC 53907

[53]

 PB1

Wild type strain, NCIMBI 13113

[53]

E. colistrains

 DH5α

General cloning host. F−thi-1 endA1 hsdR17(r− m−) supE44 ΔlacU169 (−80lacZΔM15) recA1 gyrA96 relA1

Bethesda Research Laboratories

 BL21

protein expression host. ompT hsdSB (rB− mB_) gal dcm (DE3)

Novagen

C. glutamicumstrains

 ATCC 13032

WT strain, auxotrophic for biotin

[1]

Δtal

In-frame deletion of the tal gene of WT

This study

Plasmids

 pVWEx1

KmR; C. glutamicum-E. coli shuttle vector (PtaclacIqoriVC.goriVE.c)

[46]

 pVWEx1-taPB1

derived from pVWEx1, for regulated expression of tal of B. methanolicus PB1

This study

 pVWEx1-taMGA3rec

derived from pVWEx1, for regulated expression of modified tal of B. methanolicus MGA3

This study

 pVWEx1-talCG

derived from pVWEx1, for regulated expression of tal (cg1776) of C. glutamicum ATCC 13032

This study

 pHP13

B. subtilis-E coli shuttle vector; ClmR

[22]

 pTH1mp-taPB1

pHP13 derivate with tal of B. methanolicus PB1 under control of mdh promoter

This study

 pET28b

KanR; T7lac; vector for his-tagged protein overproduction

(Novagen)

 pET28b-taPB1

purification of his-tagged B. methanolicus PB1 Ta from E. coli BL21(DE3)

This study

 pET28b-taMGA3rec

purification of his-tagged modified B. methanolicus MGA3 Ta from E. coli BL21(DE3)

This study

 pET28b-taMGA3_M1

derived from pET28b, for expression of variant M1 of taMGA3 from B. methanolicus MGA3, see Table 3

This study

 pET28b-taMGA3_M2

derived from pET28b, for expression of variant M2 of taMGA3 from B. methanolicus MGA3, see Table 3

This study

 pET28b-taMGA3_M3

derived from pET28b, for expression of variant M3 of taMGA3 from B. methanolicus MGA3, see Table 3

This study

 pET28b-taMGA3_M4

derived from pET28b, for expression of variant M4 of taMGA3 from B. methanolicus MGA3, see Table 3

This study

 pET28b-taMGA3_M5

derived from pET28b, for expression of variant M5 of taMGA3 from B. methanolicus MGA3, see Table 3

This study

 pET28b-taMGA3_M6

derived from pET28b, for expression of variant M6 of taMGA3 from B. methanolicus MGA3, see Table 3

This study

 pET28b-taMGA3

derived from pET28b, for expression of variant of the annotated taMGA3 from B. methanolicus MGA3, see Table 3

 

 pK19mobsacB

KanR; vector for gene deletions (RP4 mob; sacB B. subtilis; lacZ; OriVE.c.)

[52]

 pK19mobsacB-Δtal

derived from pK19mobsacB for in-frame deletion of C. glutamicum tal

This study

  1. Abbreviations: SpeR, spectinomycin resistance; CmR, chloramphenicol resistance; KanR kanamycin resistance