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Table 1 Biochemical properties of TaPB1

From: Transaldolase in Bacillus methanolicus: biochemical characterization and biological role in ribulose monophosphate cycle

Parameter

TaPB1

Molecular weight

54 kDa

Optimal conditions

50 mM Tris-HCl, pH 7.8, 50 °C

Optimal pH

7.2–7.4

Optimal temperature

60 °C

Temperature stability

<  60 °C

Kinetics

F6P

KM

0.74 mM

Vmax

16.3 U/mg

kcat

7.35 s−1

kcat/KM

10 s− 1 mM− 1

E4P

KM

2.5 mM

Vmax

8.9 U/mg

kcat

4 s−1

kcat/KM

1.6 s− 1 mM− 1

  1. Values for KM (mM), Vmax (U/mg), and catalytic efficiency (kcat/KM = s− 1 mM− 1) were determined for two independent protein purifications and mean values and arithmetric deviations from the mean are given
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BMC Microbiology

ISSN: 1471-2180

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