Widespread occurrence of ‘Candidatus Phytoplasma ulmi’ in elm species in Germany

BMC Microbiology

Table 2 Ct values of DNA standards after qPCR with the universal phytoplasma- and ‘Ca. P. ulmi’-specific spacer qPCR assay

Copy number/assay	Universal phytoplasma assay^a	‘Ca. P. ulmi’-specific spacer assay^a
10⁰⁸	19.7	21.6
10⁰⁷	21.4	23.2
10⁰⁶	22.5	24.3
10⁰⁵	25.7	27.1
10⁰⁴	30.0	30.4
10⁰³	31.3	32.0
10⁰²	34.5	35.4
10⁰¹	37.2	35.1

^a, Mean of four technical replicates including DNA from a non-infected elm tree

ISSN: 1471-2180