Table 2 F. tularensis actual and targeted starting concentrations for each experiment
From: Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR
Experiment | Strain | Actual Starting Concentrations for each Targeted Concentration | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Low | Mid | High | ||||||||||||||
1 CFU mL− 1 | 10 CFU mL− 1 | 100 CFU mL− 1 | ||||||||||||||
Replicates | mean | SD | Replicates | mean | SD | Replicates | mean | SD | ||||||||
Inactivated target cells | LVS | – | – | – | – | – | – | – | – | – | ||||||
IN99 | 2 | 4 | 2 | 3 | 1 | 20 | 36 | 22 | 26 | 9 | 193 | 260 | 107 | 187 | 77 | |
Schu4 | < 1 | < 1 | 1 | – | – | 8 | 8 | 8 | 8 | 0 | 48 | 51 | 37 | 45 | 7 | |
Humic Acid | LVS | 3 | 1 | 2 | 2 | 1 | 20 | 15 | 20 | 18 | 3 | 147 | 140 | 227 | 171 | 48 |
IN99 | < 1 | < 1 | < 1 | – | – | 2 | 2 | < 1 | – | – | 17 | 9 | 7 | 11 | 5 | |
Schu4 | 2 | 2 | 1 | 2 | 1 | 27 | 31 | 20 | 26 | 6 | TNTC | TNTC | TNTC | – | – | |
Arizona Test Dust | LVS | 1 | 1 | 1 | 1 | 0 | 13 | 13 | 15 | 14 | 1 | 51 | 67 | 94 | 71 | 22 |
IN99 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
Schu4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
Well Water | LVS | < 1 | < 1 | < 1 | – | – | 5 | 4 | 4 | 4 | 1 | 31 | 23 | 21 | 25 | 5 |
IN99 | 1 | 1 | 2 | 1 | 1 | 10 | 15 | 12 | 12 | 3 | 61 | 73 | 83 | 72 | 11 | |
Schu4 | 1 | 1 | 1 | 1 | 0 | 12 | 11 | 13 | 12 | 1 | 123 | 107 | 103 | 111 | 11 | |
Drinking Water | LVS | 1 | 1 | 1 | 1 | 0 | 15 | 13 | 13 | 14 | 1 | 39 | 46 | 90 | 58 | 28 |
IN99 | 4 | 4 | 4 | 4 | 0 | 33 | 42 | 46 | 40 | 7 | 287 | 400 | 300 | 329 | 62 | |
Schu4 | < 1 | 1 | < 1 | – | – | 9 | 8 | 7 | 8 | 1 | 41 | 47 | 41 | 43 | 3 | |