Table 1 Francisella spp. strains and specificity of PCR assays used in this study
From: Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR
Francisella spp. strain | Description | Source | fopA (87 bp) | pdpD (104 bp) | ISFtu2 (118 bp) |
|---|---|---|---|---|---|
F. tularensis subsp. tularensis Type A | |||||
Schu4 | Clinical isolate, Ohio, Type A1 strain | Laura Rose, CDC | + | + | – |
F2246 | Maryland isolate, Type A1 strain | Laura Rose, CDC | + | + | – |
H3563 | Oklahoma isolate, Type A1 strain | Laura Rose, CDC | + | + | – |
KC1482 | Utah isolate, Type A2, attenuated | Laura Rose, CDC | + | + | – |
F. tularensis subsp. holarctica Type B | |||||
LVS | Live vaccine strain, attenuated | Laura Rose, CDC | + | – | + |
IN99 | Indiana isolate | Laura Rose, CDC | + | – | + |
NY98 | Clinical isolate, New York | Laura Rose, CDC | + | – | + |
OR96 | Oregon isolate | Laura Rose, CDC | + | – | + |
KY99 | Kentucky isolate | Laura Rose, CDC | + | – | + |
F. novicida strain U112a | Environmental isolate, saltwater | BEI Resources | + | – | – |
F. philomiragia strain 25,015 | Isolated from moribund muskrat, Utah | ATCC | – | – | – |
F. philomiragia strain 25,018 | Water isolate, Odgen Bay Refuge, Utah | ATCC | – | – | – |