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Table 1 Francisella spp. strains and specificity of PCR assays used in this study

From: Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR

Francisella spp. strainDescriptionSourcefopA (87 bp)pdpD (104 bp)ISFtu2 (118 bp)
F. tularensis subsp. tularensis Type A
 Schu4Clinical isolate, Ohio, Type A1 strainLaura Rose, CDC++
 F2246Maryland isolate, Type A1 strainLaura Rose, CDC++
 H3563Oklahoma isolate, Type A1 strainLaura Rose, CDC++
 KC1482Utah isolate, Type A2, attenuatedLaura Rose, CDC++
F. tularensis subsp. holarctica Type B
 LVSLive vaccine strain, attenuatedLaura Rose, CDC++
 IN99Indiana isolateLaura Rose, CDC++
 NY98Clinical isolate, New YorkLaura Rose, CDC++
 OR96Oregon isolateLaura Rose, CDC++
 KY99Kentucky isolateLaura Rose, CDC++
F. novicida strain U112aEnvironmental isolate, saltwaterBEI Resources+
F. philomiragia strain 25,015Isolated from moribund muskrat, UtahATCC
F. philomiragia strain 25,018Water isolate, Odgen Bay Refuge, UtahATCC
  1. CDC Centers for Disease Control and Prevention, USA
  2. ATCC American Type Culture Collection, USA
  3. aThe following isolate was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Francisella novicida, Strain Utah 112, NR-13
  4. PCR amplicon/signal “+” detected or “-” not detected. Data are representative of three replicates
  5. Sensitivity for each assay based on standard curves was 10 cell equivalents per mL