Fig. 2
From: Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR
Detection of F. tularensis in the presence of humic acid and ATD. F. tularensis strain LVS (circles), IN99 (squares), and Schu4 (triangles) were incubated in BVFH containing either humic acid (a-d) or ATD (e-h) at targeted starting concentrations of 100 (black symbols), 10 (red symbols), and 1 (blue symbols) CFU mL− 1. Open symbols represent control wells with Milli-Q® water. Filled-in symbols represent wells with humic acid or ATD. Growth was monitored by CFU enumeration (a and e) and results of the culture-based PCR assay are shown in the bottom three rows. ΔCT of 6 is indicated by the bold black line. Circled data points are referenced in the main text