Fig. 1
From: Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR
Detection of F. tularensis in the presence of inactivated target cells. F. tularensis strain LVS (a-d), IN99 (e-h), and Schu4 (i-l) were incubated in BVFH media containing 0 (circle), 102 (square), 104 (triangle), or 106 (diamond) cells mL− 1 of their respective isopropanol-inactivated cells. For each inactivated target, culturable cells were added at targeted starting concentrations of 100 (black symbols), 10 (red symbols), and 1 (blue symbols) CFU mL− 1. Growth was monitored by CFU enumeration (a, e, and i) and results of the culture-based PCR assay are shown in the bottom three rows. ΔCT of 6 is indicated by the bold black line