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Table 4 Protocols used for conventional PCR and RT-PCR

From: Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection

StagePCRRT-PCR
 LeptoA/LeptoBL3/L4L4/LPato2 
 Temp. (°C)Time (min)Cycles (n)Temp. (°C)Time (min)Cycles (n)Temp. (°C)Time (min)Cycles (n)Temp. (°C)Time (s)Cycles (n)
Initial denaturation933193319331 600 
Denaturation931359313593135951545
Annealing6015715215310
Elongation7217217217210
Final elongation72917291729140301
Hold4  4  4