Table 3 Primers and probe sequences for polymerase chain reactions (PCR) performed in the study
From: Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection
Primer | Sequence |
|---|---|
L3 | 5′ – TgA ggg TTA AAA CCC CCA AC – 3` |
L4 | 5′ – gAT TTT TCg ggT AAA gAT T – 3` |
LeptoA | 5′ – ggC gCg TCT TAA ACA Tg – 3` |
LeptoB | 5′ – TTC CCC CCA TTg AgC AAg ATT – 3` |
LPato2 | 5′ – TCA CAT Y gCT TAT TTT – 3` |
LipL32-45F | 5′ – AAg CAT TAC CgC TTg Tgg Tg – 3’ |
LipL32-286R | 5′ – gAACTCCCATTTCAgCgATT – 3’ |
Probe LipL32-189P | 5′ – LC610-AAA gCC Agg ACA AgC gCC g--BHQ2–3’ |