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Table 1 Results for the detection of leptospiral DNA using conventional PCR and RT-PCR across 400 samples of either blood or urine

From: Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection

Conventional PCR

RT PCR

Blood

Urine

Blood & Urine

Pos (%)

Neg (%)

All (%)

Pos (%)

Neg (%)

All

Pos (%)

Neg (%)

All

Pos

16 (6.8)

0

16 (6.8)

9 (5.4)

2 (1.2)

11 (6.6)

25 (6.3)

2 (0.5)

27 (6.8)

Neg

15 (6.4)

203 (86.8)

218 (93.2)

7 (4.2)

148 (89.2)

155 (93.4)

22 (5.5)

351 (87.7)

373 (93.2)

All

31 (13.2)

203 (86,8)

234 (100)

16 (9.6)

150 (90.4)

166 (100)

47 (11.8)

353 (88.2)

400 (100)

p (Chi sq)

0.0003

0.1824

0.0001

Kappa

0.6492

0.6383

0.6453