From: Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection
Conventional PCR | RT PCR | ||||||||
---|---|---|---|---|---|---|---|---|---|
Blood | Urine | Blood & Urine | |||||||
Pos (%) | Neg (%) | All (%) | Pos (%) | Neg (%) | All | Pos (%) | Neg (%) | All | |
Pos | 16 (6.8) | 0 | 16 (6.8) | 9 (5.4) | 2 (1.2) | 11 (6.6) | 25 (6.3) | 2 (0.5) | 27 (6.8) |
Neg | 15 (6.4) | 203 (86.8) | 218 (93.2) | 7 (4.2) | 148 (89.2) | 155 (93.4) | 22 (5.5) | 351 (87.7) | 373 (93.2) |
All | 31 (13.2) | 203 (86,8) | 234 (100) | 16 (9.6) | 150 (90.4) | 166 (100) | 47 (11.8) | 353 (88.2) | 400 (100) |
p (Chi sq) | 0.0003 | 0.1824 | 0.0001 | ||||||
Kappa | 0.6492 | 0.6383 | 0.6453 |