Table 2 Bacterial strains and plasmids used in this study
Strain or plasmid | Properties | Source or Reference |
|---|---|---|
Strains | ||
A. Averonii TH0426 | wild type isolated from the yellow catfish, Ampr | This study |
∆dotU | Isogenic dotU mutant of strain TH0426 | This study |
C-dotU | Mutant ∆dotU complemented with intact dotU gene | This study |
E. coli | ||
Trans1-T1 | F-φ80(lacZ)ΔM15ΔlacX74hsdR (rk -, mk +) ΔrecA1398endA1tonA | TransGene Biotech |
DH5α-λpir | Λpir-lysogen of DH5α | Stored in our lab |
WM3064 | thrB1004 pro thi rpsL hsdS lacZ△M15RP4–1360(araBAD)567△dapA1341::[erm pir(wt)] | Stored in our lab |
Plasmids | ||
pEASY-Blunt Zero | TA cloning vector, Ampr | TransGene Biotech |
pEASY-UD dotU | Carrying the flanking region of the ORF for dotU TA cloning | This study |
pRE112 | pGP704 suicide plasmid, pir depengent, oriT, oriV, sacB, Cmr | Stored in our lab |
pRE112-UD dotU | pRE112 carrying the flanking region of the dotU ORF, Cmr | This study |
pBBR1-MCS | Broad-host-range vector, Cmr | Stored in our lab |
pBBR-dotU | pBBR carrying of 998 bp containing the promoter and dotU ORF, Cmr | This study |