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Table 2 Bacterial strains and plasmids used in this study

From: Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426

Strain or plasmid


Source or Reference


A. Averonii TH0426

wild type isolated from the yellow catfish, Ampr

This study


Isogenic dotU mutant of strain TH0426

This study


Mutant ∆dotU complemented with intact dotU gene

This study

E. coli


F-φ80(lacZ)ΔM15ΔlacX74hsdR (rk -, mk +) ΔrecA1398endA1tonA

TransGene Biotech


Λpir-lysogen of DH5α

Stored in our lab


thrB1004 pro thi rpsL hsdS lacZM15RP4–1360(araBAD)567dapA1341::[erm pir(wt)]

Stored in our lab


 pEASY-Blunt Zero

TA cloning vector, Ampr

TransGene Biotech

 pEASY-UD dotU

Carrying the flanking region of the ORF for dotU TA cloning

This study


pGP704 suicide plasmid, pir depengent, oriT, oriV, sacB, Cmr

Stored in our lab

 pRE112-UD dotU

pRE112 carrying the flanking region of the dotU ORF, Cmr

This study


Broad-host-range vector, Cmr

Stored in our lab


pBBR carrying of 998 bp containing the promoter and dotU ORF, Cmr

This study