Modified high-throughput Nile red fluorescence assay for the rapid screening of oleaginous yeasts using acetic acid as carbon source

Table 1 Relative fluorescence units (RFUs) determined by Nile red fluorimetric assay, and lipid content (% (w/w)) determined by gravimetric method after solvent extraction with n-hexane

Yeast ID	Yeast species	Lipid content (% (w/w))	RFUs (broth medium)	RFUs (solid medium)
V011	Yarrowia lipolytica	11.9 ± 0.9	9.9 ± 1.7	10.3 ± 1.7
V028	Kazachstania humilis	27.2 ± 1.7	30.3 ± 1.5	26.9 ± 2.5
V051	Pichia kudriavzevii	23.6 ± 5.8	14.8 ± 1.2	19.7 ± 1.3
V088	Wickerhamomyces anomalus	19.3 ± 2.1	17.6 ± 0.2	29.1 ± 2.7
V102	Pichia fermentans	28.4 ± 2.1	32.6 ± 0.1	31.9 ± 3.0
V124	Cyberlindnera jadinii	30.0 ± 2.4	20.4 ± 1.5	24.4 ± 0.5
V134	Apiotrichum brassicae	58.1 ± 2.3	71.1 ± 3.6	138.0 ± 18.3
V166	Pichia fermentans	22.4 ± 2.4	27.2 ± 0.5	41.4 ± 0.3
V188	Pichia fermentans	25.3 ± 0.6	25.9 ± 1.3	24.3 ± 1.3
V194	Pichia kudriavzevii	59.3 ± 0.6	85.1 ± 0.2	132.3 ± 7.1
V202	Pichia kudriavzevii	16.9 ± 4.5	10.5 ± 1.2	14.6 ± 0.5
V209	Candida tropicalis	21.7 ± 3.5	13.3 ± 2.0	54.9 ± 1.3
V211	Geotrichum candidum	13.3 ± 1.1	9.3 ± 1.4	6.7 ± 1.3
V213	Metschnikowia pulcherrima	29.6 ± 1.3	35.9 ± 1.1	42.2 ± 3.5
V247	Geotrichum candidum	16.1 ± 4.7	11.3 ± 1.2	5.6 ± 1.0
V535	Saccharomycopsis vini	26.6 ± 2.6	20.8 ± 2.2	25.2 ± 2.4
V673	Saccharomyces cerevisiae	26.0 ± 3.6	22.4 ± 1.9	18.7 ± 2.7
V674	Saccharomycopsis fibuligera	26.9 ± 0.6	19.0 ± 3.4	24.8 ± 3.2

Yeasts were cultivated during 120 h in broth and solid mineral media, with HAc 15 g/L as carbon source. Mean values of three replicates for RFUs (both in broth or solid media) did not differ significantly (p < 0.05)

ISSN: 1471-2180