Fig. 9

The inhibitive effect of Faecalibacterium prausnitzii on the growth of MCF-7 cells through IL-6. MCF-7 cells were subjected to proliferation usingCCK-8, apoptosis and invasion assays as described in the text (see Materials and Methods). MCF-7 cells were incubated with DMEM medium as control, Faecalibacterium prausnitzii supernatant (F.P), and Faecalibacterium prausnitzii supernatant plus 50 ng/ml recombinant human IL-6 (F.P + IL-6) for 72 h, respectively. Then CCK-8 (a), apoptosis (b and c) and invasion (d and e) assays were performed and the data was analyzed. # indicates control vs F.P group, P < 0.05; *indicates F.P vs F.P + IL-6 group, P < 0.05