Fig. 8

The inhibitive effect of Faecalibacterium prausnitzii on IL-6/STAT3 pathway in MCF-7 cells. a The abundance gut bacteria difference between breast cancer women group and no-breast cancer women group was detected by 16S rDNA amplicon sequencing analysis. The brown color presents the abundance gut bacteria in no-breast cancer women group and the blue color presents the abundance gut bacteria in breast cancer women group. b and c MCF-7 cells were incubated with high or low concentration of Faecalibacterium prausnitzii (F.P) supernatant, respectively for 72 h. Then cellular medium was collected and detected the secretion level of IL-6. The cells also collected to analyze the protein expression of p-JAK2, p-STAT3, total JAK2 and STAT3. d MCF-7 cells were incubated with Faecalibacterium prausnitzii (F.P) supernatant or Faecalibacterium prausnitzii (F.P) supernatant plus 50 ng/ml recombinant human IL-6, respectively for 72 h. Then cells collected to analyze the protein expression of p-JAK2, p-STAT3, total JAK3 and STAT3. * indicates control vs F.P group, P < 0.05