Table 2 Primer sets for multilocus sequence typing (MLST) analysis of Golovinomyces in this study
DNA regions | Primer | Primer sequences (5′ → 3′) | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
ITS | ITS5 ITS4 | GGAAGTAAAAGTCGTAACAAGG TCCTCCGCTTATTGATATGC | 52 | 600 | [44] |
28S rDNA | LSU1 LSU2 | ACCCGCTGAACTTAAGCATA CCTTGGTCCGTGTTTCAAGA | 52 | 500 | [45] |
IGS | IGS-12a NS1R | AGTCTGTGGATTAGTGGCCG GAGACAAGCATATGACTAC | 52 | 400 | [46] |
TUB2 | TubF1 TubR1 | AGGTTCACCTCCAGACTGG CCAGCACGAACAGCATCCAT | 52 | 450 | This study |
CHS1 | gCS1a1 gCS1b | GGTGCATTCTCGGCATATCG CGTCACCCTTGGTGCCCCAAG | 52 | 1000 | This study |