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Table 2 Cycling parameters for rhAmp anthrose assays in QuantStudio 7 flex

From: Nucleotide polymorphism assay for the identification of west African group Bacillus anthracis: a lineage lacking anthrose

Cycle

Temperature

Time

Data collection

Pre-read stage

60 °C

30 s

On

Initial denature

95 °C

3 min

Off

PCR

(40 cycles)

95 °C

10 s

Off

60 °C

30 s

Off

68 °C

40 s

On

Post-read stage

60 °C

30 s

On

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BMC Microbiology

ISSN: 1471-2180

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