Fig. 4

Evaluation of B. anthracis diversity panel. The discriminatory power of the 892 (a) and 1352 (b) genotyping assays was evaluated by testing a global panel of B. anthracis strains. The red dots along the x axis illustrate the Sterne-specific genotype (n = 44). Plasmid control replicates carrying the Sterne anthrose operon also clustered in this group (n = 3, red). The four Nigerian isolates (depicted in blue) clustered at the top left corner of the y axis along with plasmid control replicates carrying the anthrose operon from one of the western African strains (n = 3, blue). Heterozygous controls (green clusters) were tested by combining equivalent amounts of Sterne and Nigerian DNA and can help the software make appropriate determination when dealing with large panel of strains. Interestingly, UF00910 from Poland failed to amplify with either of the anthrose alleles after repeated attempts (depicted as X at plot origins). Black squares near the plot origin represent negative controls