Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria

Jia, Huaiyu; Fang, Renchi; Lin, Jie; Tian, Xuebin; Zhao, Yajie; Chen, Lijiang; Cao, Jianming; Zhou, Tieli

doi:10.1186/s12866-019-1692-3

BMC Microbiology

Table 1 Colistin MICs obtained by broth microdilution and results of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test

From: Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria

Isolate	Species	Resistant Phenotype	MIC (mg/L)	Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP Test
Isolate	Species	Resistant Phenotype	MIC (mg/L)	Result	Discrepancies with BMD MIC colistin result
BM1539	A. baumannii	R	8	Positive	No
BM1579	A. baumannii	R	4	Positive	No
BM1595	A. baumannii	R	4	Positive	No
BM2349	A. baumannii	R	4	Positive	No
BM2370	A. baumannii	R	16	Positive	No
BM2412	A. baumannii	R	4	Positive	No
BM2431	A. baumannii	R	8	Positive	No
BM2622	A. baumannii	R	8	Positive	No
TL1671	P. aeruginosa	R	4	Positive	No
TL1722	P. aeruginosa	R	4	Positive	No
TL1736	P. aeruginosa	R	≥32	Positive	No
TL1744	P. aeruginosa	R	4	Positive	No
TL2204	P. aeruginosa	R	4	Positive	No
TL2294	P. aeruginosa	R	4	Positive	No
TL2314	P. aeruginosa	R	≥32	Positive	No
TL2917	P. aeruginosa	R	4	Positive	No
TL2967	P. aeruginosa	R	4	Positive	No
TL3008	P. aeruginosa	R	16	Positive	No
TL3086	P. aeruginosa	R	≥32	Positive	No
FK20	K. pneumoniae	R	≥32	Positive	No
FK26	K. pneumoniae	R	≥32	Positive	No
FK150	K. pneumoniae	R	≥32	Positive	No
FK169	K. pneumoniae	R	≥32	Positive	No
FK171	K. pneumoniae	R	≥32	Positive	No
FK591	K. pneumoniae	R	≥32	Positive	No
FK610	K. pneumoniae	R	≥32	Positive	No
FK1342	K. pneumoniae	R	≥32	Positive	No
FK1913	K. pneumoniae	R	≥32	Positive	No
FK1986	K. pneumoniae	R	8	Positive	No
FK2066	K. pneumoniae	R	≥32	Positive	No
FK2166	K. pneumoniae	R	≥32	Positive	No
FK2778	K. pneumoniae	R	≥32	Positive	No
FK2911	K. pneumoniae	R	≥32	Positive	No
FK3789	K. pneumoniae	R	≥32	Positive	No
FK3810	K. pneumoniae	R	≥32	Positive	No
FK3994	K. pneumoniae	R	≥32	Positive	No
FK6556	K. pneumoniae	R	32	Positive	No
FK6663	K. pneumoniae	R	32	Positive	No
FK6696	K. pneumoniae	R	16	Positive	No
DC90	E. coli	R	8	Positive	No
DC2562	E. coli	R	8	Positive	No
DC3411	E. coli	R	4	Positive	No
DC3539	E. coli	R	16	Positive	No
DC3599	E. coli	R	8	Positive	No
DC3658	E. coli	R	8	Positive	No
DC3737	E. coli	R	8	Positive	No
DC3802	E. coli	R	4	Positive	No
DC3806	E. coli	R	8	Positive	No
DC3846	E. coli	R	16	Positive	No
DC4887	E. coli	R	8	Positive	No
DC5262	E. coli	R	8	Positive	No
DC5286	E. coli	R	8	Positive	No
DC7333	E. coli	R	4	Positive	No
BM1505	A. baumannii	S	0.125	Negative	No
BM1506	A. baumannii	S	0.5	Negative	No
BM1507	A. baumannii	S	0.06	Negative	No
BM1508	A. baumannii	S	0.125	Negative	No
BM1509	A. baumannii	S	0.125	Negative	No
BM1510	A. baumannii	S	0.125	Negative	No
BM1511	A. baumannii	S	0.125	Negative	No
BM1512	A. baumannii	S	0.25	Negative	No
BM1513	A. baumannii	S	0.125	Negative	No
BM1514	A. baumannii	S	0.125	Negative	No
BM4151	A. baumannii	S	0.25	Negative	No
BM4152	A. baumannii	S	0.06	Negative	No
BM4153	A. baumannii	S	0.03	Negative	No
BM4154	A. baumannii	S	0.125	Negative	No
BM4155	A. baumannii	S	0.125	Negative	No
BM4156	A. baumannii	S	0.125	Negative	No
BM4158	A. baumannii	S	0.125	Negative	No
BM4159	A. baumannii	S	0.125	Negative	No
BM4160	A. baumannii	S	0.5	Negative	No
BM4161	A. baumannii	S	0.125	Negative	No
BM4162	A. baumannii	S	0.06	Negative	No
BM4163	A. baumannii	S	0.06	Negative	No
BM4164	A. baumannii	S	0.06	Negative	No
BM4165	A. baumannii	S	0.06	Negative	No
BM4166	A. baumannii	S	0.125	Negative	No
BM4167	A. baumannii	S	0.125	Negative	No
BM4168	A. baumannii	S	0.25	Negative	No
BM4169	A. baumannii	S	0.5	Negative	No
BM4170	A. baumannii	S	0.125	Negative	No
BM4171	A. baumannii	S	0.06	Negative	No
BM4172	A. baumannii	S	≤0.06	Negative	No
BM4173	A. baumannii	S	0.06	Negative	No
BM4174	A. baumannii	S	0.06	Negative	No
BM4175	A. baumannii	S	2	Negative	No
BM4176	A. baumannii	S	0.06	Negative	No
BM4177	A. baumannii	S	0.06	Negative	No
BM4178	A. baumannii	S	0.06	Negative	No
BM4179	A. baumannii	S	0.25	Negative	No
BM4180	A. baumannii	S	0.06	Negative	No
BM4181	A. baumannii	S	0.125	Negative	No
BM4182	A. baumannii	S	0.25	Negative	No
BM4183	A. baumannii	S	1	Negative	No
BM4184	A. baumannii	S	1	Positive	Yes, ME
BM4185	A. baumannii	S	1	Negative	No
BM4186	A. baumannii	S	1	Negative	No
BM4187	A. baumannii	S	0.125	Negative	No
BM4188	A. baumannii	S	0.5	Positive	Yes, ME
BM4189	A. baumannii	S	0.5	Negative	No
BM4190	A. baumannii	S	0.125	Negative	No
BM4191	A. baumannii	S	0.5	Negative	No
TL2916	P. aeruginosa	S	0.125	Negative	No
TL2915	P. aeruginosa	S	≤0.06	Negative	No
TL2914	P. aeruginosa	S	0.125	Negative	No
TL2913	P. aeruginosa	S	0.125	Negative	No
TL2911	P. aeruginosa	S	0.125	Negative	No
TL2910	P. aeruginosa	S	0.25	Negative	No
TL2908	P. aeruginosa	S	0.125	Negative	No
TL2907	P. aeruginosa	S	0.5	Negative	No
TL2906	P. aeruginosa	S	0.125	Negative	No
TL2905	P. aeruginosa	S	0.125	Negative	No
TL2904	P. aeruginosa	S	0.125	Negative	No
TL2901	P. aeruginosa	S	0.125	Negative	No
TL2899	P. aeruginosa	S	0.125	Negative	No
TL2898	P. aeruginosa	S	0.125	Negative	No
TL2897	P. aeruginosa	S	0.125	Negative	No
TL2895	P. aeruginosa	S	0.125	Negative	No
TL2893	P. aeruginosa	S	≤0.06	Negative	No
TL2892	P. aeruginosa	S	0.125	Negative	No
TL2891	P. aeruginosa	S	0.06	Negative	No
TL2890	P. aeruginosa	S	0.25	Negative	No
TL2889	P. aeruginosa	S	0.5	Negative	No
TL2886	P. aeruginosa	S	0.5	Negative	No
TL2885	P. aeruginosa	S	0.25	Negative	No
TL2884	P. aeruginosa	S	0.25	Negative	No
TL2883	P. aeruginosa	S	0.5	Negative	No
TL2882	P. aeruginosa	S	0.25	Negative	No
TL2881	P. aeruginosa	S	0.25	Negative	No
TL2879	P. aeruginosa	S	0.25	Negative	No
TL2878	P. aeruginosa	S	0.25	Negative	No
TL2877	P. aeruginosa	S	0.25	Negative	No
TL2875	P. aeruginosa	S	0.25	Negative	No
TL2874	P. aeruginosa	S	0.25	Negative	No
TL2873	P. aeruginosa	S	1	Negative	No
TL2872	P. aeruginosa	S	0.125	Negative	No
TL2871	P. aeruginosa	S	0.25	Negative	No
TL2870	P. aeruginosa	S	2	Negative	No
TL2869	P. aeruginosa	S	0.125	Negative	No
TL2868	P. aeruginosa	S	0.125	Negative	No
TL2867	P. aeruginosa	S	0.125	Negative	No
TL2866	P. aeruginosa	S	0.125	Negative	No
TL2865	P. aeruginosa	S	0.125	Negative	No
TL2864	P. aeruginosa	S	0.25	Negative	No
TL2863	P. aeruginosa	S	≤0.06	Negative	No
TL2862	P. aeruginosa	S	0.125	Negative	No
TL2861	P. aeruginosa	S	0.25	Negative	No
TL2858	P. aeruginosa	S	0.25	Negative	No
TL2857	P. aeruginosa	S	0.25	Negative	No
TL2856	P. aeruginosa	S	0.125	Negative	No
TL2855	P. aeruginosa	S	0.25	Negative	No
TL2854	P. aeruginosa	S	0.125	Negative	No
FK3640	K. pneumoniae	S	≤0.06	Negative	No
FK3642	K. pneumoniae	S	≤0.06	Negative	No
FK3646	K. pneumoniae	S	≤0.06	Negative	No
FK3660	K. pneumoniae	S	≤0.06	Negative	No
FK3671	K. pneumoniae	S	0.125	Negative	No
FK3686	K. pneumoniae	S	0.5	Negative	No
FK3695	K. pneumoniae	S	≤0.06	Negative	No
FK3696	K. pneumoniae	S	≤0.06	Negative	No
FK3703	K. pneumoniae	S	≤0.06	Negative	No
FK3712	K. pneumoniae	S	≤0.06	Negative	No
FK3719	K. pneumoniae	S	≤0.06	Negative	No
FK3721	K. pneumoniae	S	≤0.06	Negative	No
FK3724	K. pneumoniae	S	1	Negative	No
FK3727	K. pneumoniae	S	0.5	Negative	No
FK3730	K. pneumoniae	S	≤0.06	Negative	No
FK3732	K. pneumoniae	S	0.25	Negative	No
FK3738	K. pneumoniae	S	≤0.06	Negative	No
FK3739	K. pneumoniae	S	0.125	Negative	No
FK3740	K. pneumoniae	S	≤0.06	Negative	No
FK3741	K. pneumoniae	S	≤0.06	Negative	No
FK3745	K. pneumoniae	S	0.5	Negative	No
FK3746	K. pneumoniae	S	1	Negative	No
FK3749	K. pneumoniae	S	≤0.06	Negative	No
FK3758	K. pneumoniae	S	≤0.06	Negative	No
FK3764	K. pneumoniae	S	≤0.06	Negative	No
FK3767	K. pneumoniae	S	≤0.06	Negative	No
FK3771	K. pneumoniae	S	0.5	Negative	No
FK3784	K. pneumoniae	S	≤0.06	Negative	No
FK3800	K. pneumoniae	S	0.5	Negative	No
FK3803	K. pneumoniae	S	0.25	Negative	No
FK3813	K. pneumoniae	S	≤0.06	Negative	No
FK3817	K. pneumoniae	S	≤0.06	Negative	No
FK3824	K. pneumoniae	S	0.06	Negative	No
FK3830	K. pneumoniae	S	≤0.06	Negative	No
FK3831	K. pneumoniae	S	≤0.06	Negative	No
FK3838	K. pneumoniae	S	0.5	Negative	No
FK3844	K. pneumoniae	S	0.125	Negative	No
FK3853	K. pneumoniae	S	0.125	Negative	No
FK3878	K. pneumoniae	S	0.25	Negative	No
FK3882	K. pneumoniae	S	≤0.06	Negative	No
FK3891	K. pneumoniae	S	≤0.06	Negative	No
FK3927	K. pneumoniae	S	0.125	Negative	No
FK3938	K. pneumoniae	S	0.5	Negative	No
FK3943	K. pneumoniae	S	0.06	Negative	No
FK3946	K. pneumoniae	S	0.5	Negative	No
FK3989	K. pneumoniae	S	≤0.06	Negative	No
FK3990	K. pneumoniae	S	1	Negative	No
FK3996	K. pneumoniae	S	0.125	Negative	No
FK3999	K. pneumoniae	S	≤0.06	Negative	No
FK4002	K. pneumoniae	S	≤0.06	Negative	No
DC8640	E. coli	S	0.25	Negative	No
DC8641	E. coli	S	≤0.06	Negative	No
DC8642	E. coli	S	0.125	Negative	No
DC8643	E. coli	S	0.125	Negative	No
DC8644	E. coli	S	0.5	Negative	No
DC8645	E. coli	S	≤0.06	Negative	No
DC8646	E. coli	S	≤0.06	Negative	No
DC8647	E. coli	S	≤0.06	Negative	No
DC8648	E. coli	S	≤0.06	Negative	No
DC8649	E. coli	S	0.125	Negative	No
DC8650	E. coli	S	0.06	Negative	No
DC8651	E. coli	S	0.06	Negative	No
DC8652	E. coli	S	0.125	Negative	No
DC8653	E. coli	S	0.06	Negative	No
DC8654	E. coli	S	0.06	Negative	No
DC8655	E. coli	S	0.06	Negative	No
DC8656	E. coli	S	0.125	Negative	No
DC8657	E. coli	S	≤0.06	Negative	No
DC8658	E. coli	S	0.06	Negative	No
DC8659	E. coli	S	≤0.06	Negative	No
DC8660	E. coli	S	≤0.06	Negative	No
DC8661	E. coli	S	≤0.06	Negative	No
DC8663	E. coli	S	0.125	Negative	No
DC8664	E. coli	S	2	Negative	No
DC8665	E. coli	S	≤0.06	Negative	No
DC8666	E. coli	S	0.06	Negative	No
DC8667	E. coli	S	≤0.06	Negative	No
DC8668	E. coli	S	≤0.06	Negative	No
DC8669	E. coli	S	≤0.06	Negative	No
DC8670	E. coli	S	≤0.06	Negative	No
DC8671	E. coli	S	≤0.06	Negative	No
DC8672	E. coli	S	≤0.06	Negative	No
DC8673	E. coli	S	≤0.06	Negative	No
DC8674	E. coli	S	0.06	Negative	No
DC8675	E. coli	S	≤0.06	Negative	No
DC8676	E. coli	S	≤0.06	Negative	No
DC8677	E. coli	S	≤0.06	Negative	No
DC8678	E. coli	S	≤0.06	Negative	No
DC8679	E. coli	S	≤0.06	Negative	No
DC8680	E. coli	S	0.25	Negative	No
DC8681	E. coli	S	2	Negative	No
DC8682	E. coli	S	0.125	Negative	No
DC8683	E. coli	S	≤0.06	Negative	No
DC8684	E. coli	S	≤0.06	Negative	No
DC8685	E. coli	S	≤0.06	Negative	No
DC8686	E. coli	S	0.06	Negative	No
DC8687	E. coli	S	0.06	Negative	No
DC8688	E. coli	S	0.06	Negative	No
DC8690	E. coli	S	0.06	Negative	No
DC8691	E. coli	S	0.125	Negative	No

ME major error, S susceptible, R resistant

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