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Fig. 2 | BMC Microbiology

Fig. 2

From: HpaXpm, a novel harpin of Xanthomonas phaseoli pv. manihotis, acts as an elicitor with high thermal stability, reduces disease, and promotes plant growth

Fig. 2

SDS-PAGE and western blot of GST-HpaXpm expression. a SDS-PAGE analysis of crude protein. b SDS-PAGE analysis of purified HpaXpm protein. c, Western blot of GST-HpaXpm. In A, lane M indicates the protein marker; lanes 1–8 indicate the soluble and insoluble proteins of GST-HpaXpm gene expression under different inducing conditions: 1, 2, 3, and 7 induced by 0.05 mM IPTG; 4, 5, 6, and 7 induced by 0.1 mM IPTG; 1, 4, 6, and 7 induced at 37 °C; 2, 3, 4, and 8 induced at 28 °C; 1, 3, 4, and 5 induced at 3 h; 2, 6, 7, and 8 induced at 5 h. S, soluble protein. Ins, insoluble protein. Each protein preparation (20 μl) was loaded in the gel. In B, SDS-PAGE assays of purified HpaXpm proteins (theoretical size: 13.8 kDa). In C, the western blot shows the soluble protein of GST-HpaXpm (theoretical size: 38.8 kDa) after induction for 5 h at 37 °C in 0.05 mM IPTG. GST-HpaXpm was immunoblotted with an anti-GST with antibody

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