Table 2 Summary of the detected surfactin lipopeptides extracted from MSM cultured B. amyloliquefaciens ST34, as detected using UPLC-ESI-MS
From: Exploring the antimicrobial resistance profiles of WHO critical priority list bacterial strains
Surfactin group | UPLC Rt (minutes)a | Proposed peptide sequences in surfactin group | Mono-isotopic [M] Exp/theor Mr | Protonated Specie [M + H]+ Exp/theor m/z | Sodiated Specie [M + Na]+ Exp/theor m/z |
|---|---|---|---|---|---|
Surfactin 1 (Srf1) | 10.6; 11.2 | cyclo[(C13H24O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val] cyclo[(C13H24O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val] | 993.6376 993.6403 | 994.6472 994.6481 | 1016.6265 1016.6190 |
Surfactin 2 (Srf2) | 11.0; 11.2; 11.9 | cyclo[(C14H26O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val] cyclo[(C14H26O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val] | 1007.6521 1007.6552 | 1008.6531 1008.6592 | 1030.6392 1030.6414 |
cyclo-[(C13H24O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] cyclo[(C13H24O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] acyclo-[(C13H24O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] acyclo-[(C13H24O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] | |||||
Surfactin 3 (Srf3) | 11.6; 11.7; 12.3 | cyclo[(C15H28O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Val] cyclo[(C15H28O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Val] | 1021.6693 1021.6715 | 1022.6774 1022.6769 | 1044.6517 1044.6584 |
cyclo[(C14H26O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] cyclo[(C14H26O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] acyclo-[(C14H26O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] acyclo-[(C14H26O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] | |||||
Surfactin 4 (Srf4) | 12.1; 12.2 | cyclo[(C15H28O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] cyclo[(C15H28O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] acyclo[(C15H28O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] cyclo[(C15H28O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] | 1035.6819 1035.6881 | 1036.6902 1036.6909 | 1058.6718 1058.6662 |
Surfactin 5 (Srf5) | 12.6; 12.7 | cyclo[(C16H30O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] acyclo[(C16H30O2)-L-Glu-L-Leu-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] acyclo[(C16H30O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Leu] acyclo[(C16H30O2)-L-Glu-L-Ile-D-Leu-L-Val-L-Asp-L-Leu-L-Ile] | 1049.6992 1049.7032 | 1050.7036 1050.7066 | 1072.6941 1072.6886 |