Fig. 5

Confocal scanning laser micrographs of Pseudomonas aeruginosa NCIMB 10548 biofilms treated with ceftazidime at 40 mg L^− 1. Top panels, treatment initiated at 6 h (early stage of biofilm formation); (a1) sample imaged following 18 h exposure to treatment and (a2) 42 h exposure to treatment. Bottom panels, treatment initiated at 30 h (maturing biofilm); (b1) biofilm sampled following 18 h exposure to treatment (48 h) and (b2) 42 h exposure to treatment. Main panels show XY plane, right panels show YZ slice and bottom panels show XZ slice. Green Syto 9 staining indicates live bacterial cells and red propidium iodide staining indicates dead bacterial cells