From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii
Primers | Oligonucleotide sequence (5′ → 3′)a | Use |
---|---|---|
Deletion of bfmS in A. baumannii ATCC 17978 | ||
 BFMS01F | ATCAGTTTGGTGAACGCCTACTT | Amplification of upstream region of bfmS |
 BFMS01R | AATAAAAAAAGCACCATCAGATGCGTCAGAAATCCAA | |
 BFMS02F | GGTGCTTTTTTTATTGCTTCATTTAT | Amplification of downstream region of bfmS |
 BFMS02R | CTTCACGAGGCAGACCGCCACTTACCGTTTCCAGTAT | |
Single-copy complementation of bfmS in A. baumannii ΔbfmS mutant | ||
 BFMS03F | GATCATTATTAAGGCAATCTGATTAAACTTCCTATAAGGTTGG | Amplification of bfmS coding region with its native promoter |
 BFMR03R | ATTAAAGCAGGTGATATGAAGCAATAAAAAAAGCACCTT | |
 ABaTn01F | TGGTTTGAGCAATTGACTTGG | Amplification of the upstream region of attTn7 |
 ABaTn01R | GCCTTAATAATGATCTTTTTTGAATTACT | |
 ABaTn02F | ATCACCTGCTTTAATAATTGATTGATTA | Amplification of the downstream region of attTn7 |
 ABaTn02R | GCAACACCTTCTTCACGAGGCAGACAGTCGGTTTTAGCAGACCGTAC | |
Amplification of kanamycin-resistance cassette | ||
 U1 | GTCTGCCTCGTGAAGAAGGTG | Amplification of nptI |
 U2 | GATCCGTCGACCTGCAGG |