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Table 3 Oligonucleotides used in this study

From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii

Primers

Oligonucleotide sequence (5′ → 3′)a

Use

Deletion of bfmS in A. baumannii ATCC 17978

 BFMS01F

ATCAGTTTGGTGAACGCCTACTT

Amplification of upstream region of bfmS

 BFMS01R

AATAAAAAAAGCACCATCAGATGCGTCAGAAATCCAA

 BFMS02F

GGTGCTTTTTTTATTGCTTCATTTAT

Amplification of downstream region of bfmS

 BFMS02R

CTTCACGAGGCAGACCGCCACTTACCGTTTCCAGTAT

Single-copy complementation of bfmS in A. baumannii ΔbfmS mutant

 BFMS03F

GATCATTATTAAGGCAATCTGATTAAACTTCCTATAAGGTTGG

Amplification of bfmS coding region with its native promoter

 BFMR03R

ATTAAAGCAGGTGATATGAAGCAATAAAAAAAGCACCTT

 ABaTn01F

TGGTTTGAGCAATTGACTTGG

Amplification of the upstream region of attTn7

 ABaTn01R

GCCTTAATAATGATCTTTTTTGAATTACT

 ABaTn02F

ATCACCTGCTTTAATAATTGATTGATTA

Amplification of the downstream region of attTn7

 ABaTn02R

GCAACACCTTCTTCACGAGGCAGACAGTCGGTTTTAGCAGACCGTAC

Amplification of kanamycin-resistance cassette

 U1

GTCTGCCTCGTGAAGAAGGTG

Amplification of nptI

 U2

GATCCGTCGACCTGCAGG

  1. a Underlined sequences indicate regions that are not complementary to the templates