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Table 3 Oligonucleotides used in this study

From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii

PrimersOligonucleotide sequence (5′ → 3′)aUse
Deletion of bfmS in A. baumannii ATCC 17978
 BFMS01FATCAGTTTGGTGAACGCCTACTTAmplification of upstream region of bfmS
 BFMS01RAATAAAAAAAGCACCATCAGATGCGTCAGAAATCCAA
 BFMS02FGGTGCTTTTTTTATTGCTTCATTTATAmplification of downstream region of bfmS
 BFMS02RCTTCACGAGGCAGACCGCCACTTACCGTTTCCAGTAT
Single-copy complementation of bfmS in A. baumannii ΔbfmS mutant
 BFMS03FGATCATTATTAAGGCAATCTGATTAAACTTCCTATAAGGTTGGAmplification of bfmS coding region with its native promoter
 BFMR03RATTAAAGCAGGTGATATGAAGCAATAAAAAAAGCACCTT
 ABaTn01FTGGTTTGAGCAATTGACTTGGAmplification of the upstream region of attTn7
 ABaTn01RGCCTTAATAATGATCTTTTTTGAATTACT
 ABaTn02FATCACCTGCTTTAATAATTGATTGATTAAmplification of the downstream region of attTn7
 ABaTn02RGCAACACCTTCTTCACGAGGCAGACAGTCGGTTTTAGCAGACCGTAC
Amplification of kanamycin-resistance cassette
 U1GTCTGCCTCGTGAAGAAGGTGAmplification of nptI
 U2GATCCGTCGACCTGCAGG
  1. a Underlined sequences indicate regions that are not complementary to the templates