From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii
Bacteria or plasmids | Relevant characteristicsa | Reference or source |
---|---|---|
Bacterial strains | ||
 A. baumannii |  |  |
  ATCC 17978 | Wild-type strain | ATCC |
  OH0790 | ATCC 17978 with ΔbfmS | This study |
  OH0883 | bfmS rescue in OH0790 | This study |
  HDK14 | ATCC 17978 with ΔompA | [31] |
 E. coli |  |  |
  DH5α | supE44 ΔlacU169 (Φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1; plasmid replication | [31] |
  S17-l λ pir | λ-pir lysogen; thi pro hsdR hsdM+ recA RP4–2 Tc::Mu-Km::Tn7; Tpr Smr; host for π-requiring plasmids; conjugal donor | [32] |
Plasmids | ||
 pRL27 | Tn5-RL27; oriR6K; Kmr | [33] |
 pBR322 | Cloning vector; Apr, Tcr | New England Biolabs |
 pUC4K | pUC4 with nptI; Apr, Kmr | Amersham Pharmacia Biotech |
 pHKD01 | Suicide vector; oriR6K, sacB, and Cmr | [31] |
 pOH786 | pHKD01 with ΔbfmS::nptI; Cmr, Kmr | This study |