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Table 1 Bacterial strains and plasmids used in this study

From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii

Bacteria or plasmids

Relevant characteristicsa

Reference or source

Bacterial strains

 A. baumannii

  

  ATCC 17978

Wild-type strain

ATCC

  OH0790

ATCC 17978 with ΔbfmS

This study

  OH0883

bfmS rescue in OH0790

This study

  HDK14

ATCC 17978 with ΔompA

[31]

 E. coli

  

  DH5α

supE44 ΔlacU169 (Φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1; plasmid replication

[31]

  S17-l λ pir

λ-pir lysogen; thi pro hsdR hsdM+ recA RP4–2 Tc::Mu-Km::Tn7; Tpr Smr; host for π-requiring plasmids; conjugal donor

[32]

Plasmids

 pRL27

Tn5-RL27; oriR6K; Kmr

[33]

 pBR322

Cloning vector; Apr, Tcr

New England Biolabs

 pUC4K

pUC4 with nptI; Apr, Kmr

Amersham Pharmacia Biotech

 pHKD01

Suicide vector; oriR6K, sacB, and Cmr

[31]

 pOH786

pHKD01 with ΔbfmS::nptI; Cmr, Kmr

This study

  1. a Tpr trimethoprim-resistant, Smr streptomycin-resistant, Apr ampicillin-resistant, Kmr kanamycin-resistant, Cmr chloramphenicol-resistant, Tcr tetracycline-resistant