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Table 1 Bacterial strains and plasmids used in this study

From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii

Bacteria or plasmidsRelevant characteristicsaReference or source
Bacterial strains
A. baumannii  
  ATCC 17978Wild-type strainATCC
  OH0790ATCC 17978 with ΔbfmSThis study
  OH0883bfmS rescue in OH0790This study
  HDK14ATCC 17978 with ΔompA[31]
E. coli  
  DH5αsupE44 ΔlacU169 (Φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1; plasmid replication[31]
  S17-l λ pirλ-pir lysogen; thi pro hsdR hsdM+ recA RP4–2 Tc::Mu-Km::Tn7; Tpr Smr; host for π-requiring plasmids; conjugal donor[32]
 pRL27Tn5-RL27; oriR6K; Kmr[33]
 pBR322Cloning vector; Apr, TcrNew England Biolabs
 pUC4KpUC4 with nptI; Apr, KmrAmersham Pharmacia Biotech
 pHKD01Suicide vector; oriR6K, sacB, and Cmr[31]
 pOH786pHKD01 with ΔbfmS::nptI; Cmr, KmrThis study
  1. a Tpr trimethoprim-resistant, Smr streptomycin-resistant, Apr ampicillin-resistant, Kmr kanamycin-resistant, Cmr chloramphenicol-resistant, Tcr tetracycline-resistant