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Fig. 6 | BMC Microbiology

Fig. 6

From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii

Fig. 6

Pathogenic effect of OMVs derived from three A. baumannii strains. a Biofilm formation of A. baumannii strains cultured with OMVs from different A. baumannii strains. A. baumannii was inoculated in polystyrene tubes and then OMVs (5 μg/ml) obtained from different A. baumannii strains were added to bacterial culture media. Biofilms formed on polystyrene tubes were stained with crystal violet. The amount of crystal violet eluted from the biofilms with ethanol was quantified as the OD570 normalized to total bacterial growth (OD600). The data are presented as mean ± SD of three independent experiments. b Cytotoxicity of A549 cells treated with OMVs from A. baumannii strains. Cells were treated with various concentrations of A. baumannii OMVs for 24 h. Cell viability was determined using the MTT assay. Data are presented as mean ± SD of three independent experiments. + p < 0.05 compared to untreated control cells. * p < 0.05 comparing the same concentration of OMVs from A. baumannii ATCC 17978

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