Fig. 5
From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii
OMV production and its protein profile in A. baumannii strains. (a and b) Production of OMVs from A. baumannii strains. OMVs were isolated from A. baumannii cultured in LB broth. a The size and number of OMV particles isolated from three A. baumannii strains were determined using nanoparticle tracking analysis. The data are representative of three independent experiments with similar results. b The protein concentration of OMVs isolated from 1 L of bacterial culture was measured using a modified BCA assay. The data are presented as mean ± SD of two independent experiments. ** p < 0.01 compared to wild-type ATCC 17978. c SDS-PAGE and western blot analyses of OMV proteins. Protein samples were resolved by SDS-PAGE in 12% gels, transferred to nitrocellulose membranes, and immunoblotted with a polyclonal anti-rabbit OmpA immune sera. Lane MW, molecular weight marker; 1, A. baumannii ATCC 17978; 2, ΔbfmS mutant OH0790; 3, ΔbfmS-complemented OH0883