Fig. 2

Production of OmpA protein and expression of ompA gene in A. baumannii strains. a SDS-PAGE analysis of bacterial proteins. The bacterial lysates and outer membrane fractions corresponding to 10 μg of protein were separated on a 12% SDS-PAGE gel. Proteins precipitated from the culture supernatants (200 ml) were resuspended in 200 μl of PBS and then 15 μl of the samples were separated on 12% SDS-PAGE gel. Lane MW, molecular weight marker; 1, A. baumannii ATCC 17978; 2, ΔbfmS mutant OH0790; 3, ΔbfmS-complemented OH0883. b Transcription levels of ompA in the three A. baumannii strains were determined using qPCR. The data are mean ± SD expression levels of the target gene in each strain relative to expression of this gene in A. baumannii ATCC 17978. Data were obtained from three independent experiments