Fig. 1
From: The sensor kinase BfmS controls production of outer membrane vesicles in Acinetobacter baumannii
Production of OmpA in the outer membrane fraction of transposon-inserted A. baumannii mutant strains. Bacteria were cultured in LB broth for 24 h and proteins (10 μg) in the outer membrane fractions were separated on a 12% SDS-PAGE gel. MW, molecular weight marker; WT, A. baumannii ATCC 17978; #691 and #692 mutant strains, Transposon was inserted in the open reading frame of the A1S_0749 (bfmS) gene. Western blot analysis was performed to identify 38 kDa-OmpA. Protein samples resolved on 12% SDS-PAGE gel were transferred to nitrocellulose membranes and immunoblotted with a polyclonal anti-rabbit OmpA immune sera