Table 1 PRM validated differentially expressed proteins in wuyiencin-treated B. cinerea
From: iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin
Accession No. | Description | Molecular Function | Biological Process | iTRAQ | PRM | ||
|---|---|---|---|---|---|---|---|
FC | p-Value | FC | p-Value | ||||
Bcin03p07210.1 | Putative alpha beta hydrolase fold protein | Hydrolase activity | Metabolic process | 4.862 | 0.001 | 7.118 | 0.035 |
Bcin01p10150.1 | Similar to phosphatidylserine decarboxylase | Phosphatidylserine decarboxylase activity | Phospholipid biosynthetic process | 3.621 | 0.004 | 4.352 | 0.004 |
Bcin10p00030.1 | Putative dimeric alpha-beta barrel protein | Secretase and lipase activity | Virulence/adaptation | 3.450 | 0.003 | 4.317 | 0.007 |
Bcin12p02040.1 | Similar to aspartic protease (Secreted protein) | Inactive proenzyme (pepsin family) | Cell wall stabilization | 2.850 | 0.011 | 3.885 | 0.041 |
Bcin12p06180.1 | Cyanide hydratase | Cyanide hydratase activity, hydrolase activity (acting on carbon-nitrogen but not peptide bonds) | Cyanide catabolic process | 3.004 | 0.006 | 3.422 | 0.016 |
Bcin15p00520.1 | Esterase | Hydrolysis enzyme | Cell membrane permeabilization | 3.514 | 0.007 | 2.938 | 0.005 |
Bcin17p00040.1 | Putative prolyl aminopeptidase protein | Aminopeptidase activity | Protein synthesis, assembly, fate and degradation | 2.637 | 0.001 | 2.853 | 0.013 |
Bcin01p10140.1 | Similar to flavin-nucleotide-binding protein | Co-factor binding; hydrolase activity | Oxidative metabolism | 4.776 | 0.013 | 2.593 | 0.016 |
Bcin04p01400.1 | Similar to short-chain dehydrogenase/reductase sdr | Oxidoreductase activity | Integral membrane protein | 2.909 | 0.001 | 2.537 | 0.031 |
Bcin11p02720.1 | Putative glycerol dehydrogenase protein | Oxidoreductase activity | Metal ion binding (NAD,zinc) | 3.317 | 0.001 | 2.489 | 0.004 |
Bcin06p00620.1 | Putative tripeptidyl peptidase a protein | Serine-type endopepidase activity | Metal ion binding (Ca2+ cofactor) | 4.850 | 0.019 | 2.389 | 0.022 |
Bcin07p04810.2 | Beta-hexosaminidase | Hydrolysis enzyme | Carbohydrate metabolism | 2.668 | 0.027 | 1.838 | 0.015 |
Bcin12p03390.1 | Glucoamylase | Glucan 1,4-alpha-glucosidase activity | Polysaccharide catabolic process | 3.160 | 0.001 | 1.703 | 0.043 |
Bcin01p08110.1 | Alpha-galactosidase | Lysosomal enzyme | Carbohydrate metabolism | 2.651 | 0.038 | 1.418 | 0.004 |
Bcin10p01350.1 | Similar to short-chain dehydrogenase/reductase SDR | Oxidore ductase activity | Integral membrane protein | 0.195 | 0.001 | 0.087 | 0.004 |
Bcin03p06060.1 | Inosine triphosphate pyrophosphatase | Metal ion binding; nucleoside riphosphate diphosphate activity, NADH pyrophosphatase activity, nucleotide binding | Deoxyribonudeoside triphosphate catabolic process; nucleotide meabolism | 0.269 | 0.001 | 0.119 | 0.005 |
Bcin02p04380.1 | Putative monooxygenase fad-binding protein | FAD; monooxygenase activity | ABA biosynthesis | 0.169 | 0.003 | 0.123 | 0.004 |
Bcin13p01010.1 | Similar to aflatoxin biosynthesis ketoreductase nor-1 | Oxidoreductase activity | Aflatoxin biosynthesis | 0.172 | 0.001 | 0.161 | 0.004 |
Bcin04p05700.1 | Putative nadp-dependent alcohol dehydrogenase protein | Alcohol dehydrogenase (NADP+) activity; zinc ion binding | NADP/NADPH balance | 0.328 | 0.007 | 0.174 | 0.026 |
Bcin06p00530.1 | Putative nadp-dependent l-serine l-allo-threonine dehydrogenase ydfg protein | Oxidoreductase activity | Amino acid catabolism and transport | 0.201 | 0.001 | 0.180 | 0.009 |
Bcin06p07160.1 | Putative peptidase s58 protein | Serine peptidase | Cell cycle regulation | 0.185 | 0.001 | 0.187 | 0.001 |
Bcin06p01180.1 | Putative catalase isozyme p protein | Catalase-peroxidase activity | Oxidative stress response | 0.214 | 0.006 | 0.222 | 0.027 |
Bcin10p05150.1 | Eukaryotic translation initiation factor 6 | Free ribosomal binding | Inhibits ribosomal subunit binding; inhibits cell growth | 0.268 | 0.001 | 0.291 | 0.042 |
Bcin03p09280.1 | Putative saccharopine dehydrogenase protein | Oxidoreductase activity | Lysine biosynthesis | 0.226 | 0.001 | 0.305 | 0.016 |
Bcin03p04480.1 | Similar to oxidoreductase | Oxidoreductase activity | Stress adaptation | 0.235 | 0.004 | 0.430 | 0.031 |
Bcin03p00400.1 | Similar to GNAT family acetyltransferase | Heme binding; oxidoreductase oxygen binding | FAD/NAD(P)-binding domain protein | 0.208 | 0.001 | 0.461 | 0.037 |
Bcin14p00610.5 | Endopolygalacturonase 2 | Polygalacturonase activity | Carbohydrate metabolism; cell wall organization | 0.150 | 0.001 | 0.653 | 0.034 |