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Fig. 2 | BMC Microbiology

Fig. 2

From: An improved bind-n-seq strategy to determine protein-DNA interactions validated using the bacterial transcriptional regulator YipR

Fig. 2

Protein purification of YipR, DNA-protein enrichment and identification of DNA binding motifs for YipR. a SDS-PAGE of the YipR protein purified by nickel affinity chromatography shows a single band of the expected size of 81 kDa (b) Assessment of enrichment of DNA recovered from Bind-n-seq reactions using real-time PCR. Samples derived from oligo only were used as positive control, No Template Control (NTC) was also included. c Quality analysis of synthesised 93-mer oligo

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