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Table 4 Sequence of primers used for each bacterial group

From: Method comparison for the direct enumeration of bacterial species using a chemostat model of the human colon

Target

Primer

Sequence (5′-3′)

Product size (bp)

Annealing (time, temp)

Reference

Bacteroides spp.

Bac303F

GAAGGTCCCCCACATTG

419

45 s, 54 °C

[34]

Bac708R

CAATCGGAGTTCTTCGTG

Enterobacteriaceae

Eco1457F

CATTGACGTTACCCGCAGAAGAAGC

190

45 s, 60 °C

[22]

Eco1652

CTCTACGAGACTCAAGCTTGC

C. leptum group

Sg-clept-F

GCACAAGCAGTGGAGT

241

45 s, 54 °C

[20]

Sg-clept-R

CTTCCTCCGTTTGTCAA

C. coccoides group

Eub338F

ACTCCTACGGGAGGCAGC

139

30s, 56 °C

[21]

Erec482R

GCTTCTTAGTCANGTACCG

Prevotella spp.

CFB286F

GTAGGGGTTCTGAGAGGA

446

30s, 56 °C

[21]

CFB719R

AGCTGCCTTCGCAATCGG

Eubacteria

8F

AGTTTGATCCTGGCTCAG

417

45 s, 54 °C

[35]

515R

GNATTACCGCGGCNGCTG

Probe

338P

FAM GCTGCCTCCCGTAGGAGT BHQ1

 

Bifidobacterium spp.

Bif551F

CGCGTCNGGTGTGAAAG

244

20s, 55 °C

[36]

Bif794R

CCCCACATCCAGCATCCA

Lactobacillus spp.

Lacto-F

GAGGCAGCAGTAGGGAATCTTC

126

45 s, 60 °C

[36]

Lacto-R

GGCCAGTTACTACCTCTATCCTTCTTC

Enterococcus spp.

Enter-F

CCCTTATTGTTAGTTGCCATCATT

144

20s, 56 °C

[23]

Enter-R

ACTCGTTGTACTTCCCATTGT

C. difficile (16S gene)

Cdiff-F

CAAGTTGAGCGATTTACTTCGGTAA

177

20s, 59 °C

[37]

Cdiff-R

CTAATCAGACGCGGGTCCAT

C. difficile (GluD gene)

GluD-F

ATGCAGTAGGGCCAACAAAA

135

20s, 55 °C

[38]

GluD-R

TTCCACCTTTACCTCCACCA

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BMC Microbiology

ISSN: 1471-2180

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