Fig. 3
From: In vitro synergy between sodium deoxycholate and furazolidone against enterobacteria
Effect of the ΔtolC and ΔacrA mutations on DOC synergy with FZ, NIT, NFZ and CM4 in E. coli. Isobolograms characterizing interactions of DOC with FZ (a), NIT (b), NFZ (c) and CM4 (d) in growth inhibition assays of the E. coli K12 strain K1508 (WT or wild-type and two isogenic deletion mutants, ΔacrA and ΔtolC). Each data point corresponds to the FIC (ratios of the 90% growth inhibition concentrations in combination vs. alone) for one of the four nitrofurans (y axis) and DOC (x axis). The ΔtolC strain (K2403) had the MICs for FZ, NIT, NFZ, CM4 at 1.25 μg/mL, 4 μg/mL, 8 μg/mL and 4 μg/mL, respectively. The ΔacrA strain (K2424) had the MICs for FZ, NIT, NFZ, CM4 at 2.5 μg/ml, 8 μg/mL, 8 μg/mL and 8 μg/mL, respectively. The WT strain K1508 had the MICs for FZ, NIT, NFZ, CM4 at 2.5, 32, 16 and 32 μg/mL, respectively