Role of membrane compartment occupied by Can1 (MCC) and eisosome subdomains in plant pathogenicity of the necrotrophic fungus Alternaria brassicicola

Table 1 Homologous proteins in A. brassicicola of the main S. cerevisiae MCC/eisosome proteins and their gene expression in response to applied treatments. Brassinin, camalexin, sorbitol and desiccation treatments were compared to non-treated cultures at two time points (0.5 h and 2 h, or 1 h and 4 h). Genes with a P-values ≤0.01 and a log2 ratio ≥ 0.5 or ≤ − 0.5 were considered as differentially expressed. Values corresponds to log2 ratio

	Protein	A. brassicicola ID	S. cerevisiae homolog (% identity)	Function	Reference	Exposure to brassinin		Exposure to camalexin		Exposure to sorbitol		Exposure to dehydration
Eisosome						0.5 h	2 h	0.5 h	2 h	0.5 h	2 h	1 h	4 h
	Pil1A	AB08863.1	YGR086C (73%)	BAR domain	[10]	0.88		0.62		2.27	0.88	1.38	1.59
	Pil1B	AB09572.1	YPL004C (50%)	BAR domain	[10]
	Slm1	AB02000.1	YIL105C (37%)	BAR domain and PH domain	[38]	2.01	0.9	1.6		1.55		2.48	1.74
	Slm2	AB05189.1	YNL047C (24%)	BAR domain and PH domain	[38]							1.18	0.61
	Pkh1	AB04691.1	YDR490C (50%)	Ser/Thr protein kinase	[14, 20]
	Pkh2	AB07167.1	YGR092W (55%)	Ser/Thr protein kinase	[14, 20]
	Pst2	AB10448.1	YCR004C (51%)	flavodoxin-like proteins	[36]
	Mdg1	AB06956.1	YHR146W (38%)	Unknown	[36]	0.85
	Ycp4	AB02777.1	YCR004C / YDR032C (57%)	flavodoxin-like proteins	[36]						0.79
	Xrn1	AB10419.1	YGL173C (63%)	Exonuclease	[37]
MCC	Sur7	AB08885.1	YML052W (34%)	Sur7 family tetraspan	[4, 6]	1.5		0.98		1.72		1.91	1.81
	Pun1	AB03912.1	YLR414C (26%)	Sur7 family tetraspan	[36]					1.39	0.94
	Nce102	AB04716.1	YPR149W (37%)	Nce102 family tetraspan	[36]	1.59		1.05		1.61		1.61	1.66
	Can1	AB08516.1	YEL063C (43%)	H⁺-driven Arg permease	[6]		−0.96			−0.8	−0.61	− 0.96
	Fur4	AB04392.1	YIR028W (46%)	H⁺-driven uracil permease	[5]								−1.06
	Tat2	AB08109.1	YKR039W (52%)	H⁺-driven Trp and Tyr permease	[14]

ISSN: 1471-2180