The Moraxella catarrhalis phase-variable DNA methyltransferase ModM3 is an epigenetic regulator that affects bacterial survival in an in vivo model of otitis media

Table 1 SMRT methylome analysis of 195ME modM3 on, modM3 off and ∆modM3

Methyltransferase specificity	Modified base	% of sites in genome methylated			Assignment^a
Methyltransferase specificity	Modified base	modM3 ON	modM3 OFF	∆modM3	Assignment^a
5′-ACATC-3′	m6A	100	0	0	M.Mca195I
5′-GCGCGC-3′	m5C	NC	NC	NC	M.Mca195ORF6035P
5′-GCYGC-3′	m5C	NC	NC	NC	M.Mca195ORF8410P

^aAssignment of methyltransferases to a methyltransferase specificity sequence is based on experimental evidence for M.Mca195I, and homology for M.Mca195ORF6035P and M.Mca195ORF8410P. M.Mca195ORF6035P shares 63% amino acid identity with M.HinP1I that recognizes the sequence 5′-GCGC-3′, and M.Mca195ORF8410P shares 71% amino acid identity with M.CocII that recognizes 5′-GCNGC-3′. Due to the low kinetic signal of 5mC methylation, the percentage of methylated sites are not calculated (NC)

ISSN: 1471-2180