Fig. 1

Establishment of P. aeruginosa wound infection. Bacteria (2.5 × 10⁶ CFU) were inoculated into each skin surgical wound. The inoculated mice were sacrificed at 4, 24, and 48 h post-infection. Serial dilutions of tissue homogenates were cultured on LB agar and incubated at 37 °C overnight followed by enumeration of bacterial colony-forming units (CFU). a _Log10 CFU/g of wounded skin tissue. b Log₁₀ CFU/g of liver tissue. Data are presented as average ± standard deviations (SD, n = 4). Different lowercase letters (a, b, c) above the bars show significant differences (p < 0.05) among different time points. Each letter represents a level of significant difference