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Fig. 2 | BMC Microbiology

Fig. 2

From: Evaluation of a new Rapid Antimicrobial Susceptibility system for Gram-negative and Gram-positive bloodstream infections: speed and accuracy of Alfred 60AST

Fig. 2

Flowchart showing laboratory workflow followed along the rapid susceptibility system (Alfred 60 AST) evaluation. Once a blood culture bottle flagged positive following incubation, Gram-stain was performed in order to distinguish between Gram-positive and Gram-negative microorganism and exclude the polymicrobial ones, which will allow to select the appropriate antibiotic panel and a direct blood sample from the bottle was loaded onto the rapid system. In parallel, the identification results were obtained by MALDI-TOF, performed from isolated colonies obtained by 4 h agar plate subculture. The mean total time from positive blood culture to identification and susceptibility results was 6 h (approx)

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