Fig. 5

Overview of three Wolbachia pseudogenes carrying deletions of various sizes. The 16S rRNA,ftsZ and wsp gene fragments of Wolbachia chromosomal insertions sequenced from natural Bactrocera and Zeugodacus populations aligned with the corresponding regions of strain wMel and Wolbachia strains infecting Bactrocera flies (cytoplasmic). Grey lines represent the deletion region. The black numbers show the positions before and after the deletions in respect to thewMel genome. The red arrows and numbers indicate the size of deletion in base pairs. Variants exhibit small number of SNPs. aVariant 1a: B. zonata (Bangladesh, Rajshahi, 03.3B), B. correcta (India, Trombay, 1.4), B. dorsalis (India, Palampur, 02.11D), B. nigrofemoralis (India, Palampur, 02.10G),B. zonata (India, Trombay, 2.2). Variant 1b:B. dorsalis (Bangladesh, Rajshahi, 03.7D), B. dorsalis (India, Palampur, 02.11D), B. zonata (India, Trombay, 8.2). Variant 1c: B. correcta (India, Trombay, 1.4).Variant 2: Z. cucurbitae (Bangladesh, Dinajpur, 07.10H). b Deletions in the ftsZ gene were identified in two B. zonata samples, B. zonata (India, Trombay, 2.2) and B. zonata (India, Trombay, 8.2). Sample 8.2 carried two different types of deletions. (C) B. zonata (India, Trombay, 2.2) contained wsp pseudogenes with two different types of deletions