Table 1 Analysis of V6-V9 16S rRNA gene sequences obtained from DGGE profiles and sequencing
DGGE Band Number | Taxonomic group (RDP) | Closest related sequence (BLAST) (Genbank Accession number) | Nucleotide bases compared | Similarity |
|---|---|---|---|---|
3 S + P Lab / 4 S + P Lab / 5 S + P Lab | Gamaproteobacteria/ Enterobacteriales/ Enterobacteriaceae/ unclassified_Enterobacteriaceae | Raoultella planticola strain Ns8 (MG544105.1) Serratia marcescens subsp. marcescens (HG326223.1) | 389 | 99% |
4 S + P Wild / 5 S Lab | Gamaproteobacteria/ Enterobacteriales/ Enterobacteriaceae/ unclassified_Enterobacteriaceae | Enterobacter soli strain YHBG2 (MG516168.1) Serratia marcescens subsp. marcescens (MG516113.1) | 384 | 100% |
1 S + P + AB Wild / 2 S + P + AB Lab | Gamaproteobacteria/ Xanthomonadales/ Xanthomonadaceae/ Stenotrophomonas | Stenotrophomonas maltophilia (MG546679.1) Stenotrophomonas maltophilia (MG546678.1) | 388 | 100% |
5 S + P Wild / 6 S + P Wild | Gamaproteobacteria/ Enterobacteriales/ Enterobacteriaceae/ unclassified _Enterobacteriaceae | Uncultured Enterobacter sp. clone 03 (KJ526996.1) Klebsiella aerogenes strain JMB006 (MG546216.1) | 387 | 99% |
10 S + P + AB Wild / 5 S + AB Wild / 1 S + P + AB Lab / 4 S + AB Lab | Alphaproteobacteria/ unclassified_Alpha-proteobacteria | Uncultured alpha proteobacterium (HM111616.1) | 390 | 99% |
3 S Wild | Gamaproteobacteria/Enterobacteriales/ Enterobacteriaceae/Citrobacter | Klebsiella sp. M5al (CP020657.1) Klebsiella oxytoca strain FCX2 16S (KU942497.1) | 387 | 100% |