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Table 1 Microscopy observations of the effect of Ab on epithelial cells at different MOIs

From: Effect of calcium on the interaction of Acinetobacter baumannii with human respiratory epithelial cells

Co-culture time pointControl group aExperimental group b
MOI 1MOI 10MOI 50MOI 100
2 h+ / -+ (−) / ±(−)+ (−) / ++ (−)+ (+) / & (+)+ (+) / & (+)
4 h++ / -++ (−) / + (−)++ (±) / +++ (−)++ (++) / && (++)++ (++) / && (++)
6 h+++ / -+++ (−) / ++ (−)+++ (+) / ++++ (−)+++ (+++) / &&& (+++)+++ (+++) / &&& (+++)
8 h++++ / -++++ (−) / ++ (−)++++ (+) / ++++ (±)+++ (+++) / &&&& (+++)+++ (+++) / &&&& (+++)
  1. Control: 0.45% NaCl
  2. a: epithelial cells / bacteria; b: epithelial cells (bubble-like dead cells) / bacteria (bacterial aggregation phenomenon)
  3. +: indicated the degree (the epithelial cells were expressed as the situation of adherent growth, bacteria were expressed as quantity, bubble-like dead cells and bacterial aggregation phenomenon were expressed as visibility); &: indicated the degree > ++++; −: indicated none
  4. For example: + / - indicated that a few of epithelial cells adhered to plates without bacteria; +++ (+++) / &&& (+++) indicated that the bacterial number increased and the bacterial aggregation phenomenon became more obvious compared to 4 h, a lot of epithelial cells adhered to plates and the bubble-like dead cells with bacterial adhesion visible