Co-culture time point | Control group a | Experimental group b |
|---|
MOI 1 | MOI 10 | MOI 50 | MOI 100 |
|---|
2 h | + / - | + (−) / ±(−) | + (−) / ++ (−) | + (+) / & (+) | + (+) / & (+) |
4 h | ++ / - | ++ (−) / + (−) | ++ (±) / +++ (−) | ++ (++) / && (++) | ++ (++) / && (++) |
6 h | +++ / - | +++ (−) / ++ (−) | +++ (+) / ++++ (−) | +++ (+++) / &&& (+++) | +++ (+++) / &&& (+++) |
8 h | ++++ / - | ++++ (−) / ++ (−) | ++++ (+) / ++++ (±) | +++ (+++) / &&&& (+++) | +++ (+++) / &&&& (+++) |
- Control: 0.45% NaCl
- a: epithelial cells / bacteria; b: epithelial cells (bubble-like dead cells) / bacteria (bacterial aggregation phenomenon)
- +: indicated the degree (the epithelial cells were expressed as the situation of adherent growth, bacteria were expressed as quantity, bubble-like dead cells and bacterial aggregation phenomenon were expressed as visibility); &: indicated the degree > ++++; −: indicated none
- For example: + / - indicated that a few of epithelial cells adhered to plates without bacteria; +++ (+++) / &&& (+++) indicated that the bacterial number increased and the bacterial aggregation phenomenon became more obvious compared to 4 h, a lot of epithelial cells adhered to plates and the bubble-like dead cells with bacterial adhesion visible
