Fig. 3

Gentamicin-based pTOR vectors enable super-transformation of G418-resistant P. palmivora strains. a A transgenic P. palmivora LILI-YKDEL strain was transformed with a gentamicin-based vector carrying a construct for constitutive expression of a Lifeact:mScarlet-I reporter. b Representative pictures of hyphae grown axenically on V8 plate containing 100 mg/L gentamicin and G418. c The same strain was transformed with a gentamicin-based vector carrying a construct for constitutive expression of a nuclear-localized mTFP1 in addition to a cytoplasmic tdTomato marker. d Representative pictures of hyphae grown axenically on V8 plate containing 100 mg/L gentamicin and G418. Arrowheads indicate nuclei. Scale bar is 10 μm