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Fig. 3 | BMC Microbiology

Fig. 3

From: N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans

Fig. 3

Gentamicin-based pTOR vectors enable super-transformation of G418-resistant P. palmivora strains. a A transgenic P. palmivora LILI-YKDEL strain was transformed with a gentamicin-based vector carrying a construct for constitutive expression of a Lifeact:mScarlet-I reporter. b Representative pictures of hyphae grown axenically on V8 plate containing 100 mg/L gentamicin and G418. c The same strain was transformed with a gentamicin-based vector carrying a construct for constitutive expression of a nuclear-localized mTFP1 in addition to a cytoplasmic tdTomato marker. d Representative pictures of hyphae grown axenically on V8 plate containing 100 mg/L gentamicin and G418. Arrowheads indicate nuclei. Scale bar is 10 μm

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BMC Microbiology

ISSN: 1471-2180

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