Fig. 2

Gentamicin is a reliable selectable marker for Phytophthora (a-b) Representative pictures of 5-day-old transgenic P. palmivora LILI (c) or 10-day-old transgenic P. infestans (d) carrying a construct for constitutive expression of the tdTomato fluorescent protein. Plates were grown on V8 or RSA, respectively, and supplemented or not with 100 mg/L gentamicin. c Representative pictures of 5-day-old transgenic P. palmivora LILI transformed with a gentamicin-based pTOR vector, grown on V8 plates containing either no antibiotic (left), 100 mg/L gentamicin (middle) or 100 mg/L geneticin (G418, right). d Representative pictures of infectious hyphae from a gentamicin-resistant P. palmivora strain expressing a constitutive Lifeact:mCitrine reporter, 24 h after infection of a Nicotiana benthamiana leaf. Arrowheads indicate haustoria. Scale bar is 10 μm